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Gene Expression Data
Assay Details
Assay
Reference: J:214045 Audouard E, et al., The Onecut transcription factor HNF-6 contributes to proper reorganization of Purkinje cells during postnatal cerebellum development. Mol Cell Neurosci. 2013 Sep;56:159-68
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6490613
Gene symbol: Onecut1
Gene name: one cut domain, family member 1
Antibody: Anti-HNF-6 (H-100) sc-13050
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Antibody name MGI:6490604 may have been used in this assay. Non-homozygous mutant animals were either wildtype or heterozygous.
Results
Specimen 2A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: metencephalon Present Single cells 2A Expression in cells in the vicinity of the cerebellum.
TS20: cerebellum primordium Absent 2A Expressing cells not located within cerebellum.
Specimen 2B: embryonic day 12.5 (more )
Note: Triple labeled: red - HNF6 (Onecut1); green - IA1 (Insm1 assay MGI:6490614); blue - Lhx1 (assay MGI:6490615 and Lhx5 (assay MGI:6490617).
Structure Level Pattern Image Note
TS20: cerebellum primordium Absent 2B No co-expression with markers of early Purkinje cells or granule cells.
Specimen 2B inset: embryonic day 12.5 (more )
Note: Triple labeled: red - HNF6 (Onecut1); green - IA1 (Insm1 assay MGI:6490614); blue - Lhx1 (assay MGI:6490615 and Lhx5 (assay MGI:6490617).
Structure Level Pattern Image Note
TS20: cerebellum primordium Absent 2B inset No co-expression with markers of early Purkinje cells or granule cells.
Specimen 2C: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: cerebellum Absent 2C
Specimen 2D: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: metencephalon Present Regionally restricted 2D Expression in vicinity of cerebellar peduncle.
Specimen 2E: embryonic day 18.5 (more )
Note: Triple labeled: red - HNF6 (Onecut1); green - IA1 (Insm1 assay MGI:6490614); blue - Lhx1 (assay MGI:6490615 and Lhx5 (assay MGI:6490617).
Structure Level Pattern Image Note
TS26: cerebellum Absent 2E
Specimen 2E inset: embryonic day 18.5 (more )
Note: Triple labeled: red - HNF6 (Onecut1); green - IA1 (Insm1 assay MGI:6490614); blue - Lhx1 (assay MGI:6490615 and Lhx5 (assay MGI:6490617).
Structure Level Pattern Image Note
TS26: metencephalon Present Regionally restricted 2E inset Expression in vicinity of cerebellar peduncle.
Specimen 2F: embryonic day 18.5 (more )
Note: Triple labeled: blue - Hnf6 (Onecut1); red - Tbr1 (assay MGI:6490618); green - Brn3 (Pou4f1 assay MGI:6490620).
Structure Level Pattern Image Note
TS26: cerebellum Absent 2F No expression in direct afferent and efferent structures of the cerebellum.
Specimen 2G: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: cerebellum Purkinje cell layer Weak 2G Low level of expression in cerebellum.
Specimen 2H: postnatal day 4 (more )
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present 2H
Specimen 2I: postnatal day 7 (more )
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Weak 2I Expression detectable until P7.
Specimen 2J: postnatal day 14 (more )
Structure Level Pattern Image Note
TS28: cerebellum Absent 2J Expression undetectable at P14.
Specimen 2G inset: postnatal day 0; Onecut1tm1Flem/Onecut1tm1Flem (more )
Structure Level Pattern Image Note
TS27: cerebellum Absent 2G inset
Specimen 2H inset: postnatal day 4; Onecut1tm1Flem/Onecut1tm1Flem (more )
Structure Level Pattern Image Note
TS28: cerebellum Absent 2H inset
Specimen 2I inset: postnatal day 7; Onecut1tm1Flem/Onecut1tm1Flem (more )
Structure Level Pattern Image Note
TS28: cerebellum Absent 2I inset
Specimen 2J inset: postnatal day 14; Onecut1tm1Flem/Onecut1tm1Flem (more )
Structure Level Pattern Image Note
TS28: cerebellum Absent 2J inset
Specimen 2L inset: postnatal day 0 (more )
Note: Double labeled: green - HNF6 (Onecut1); red - Calb1 (assay MGI:6490621);
Structure Level Pattern Image Note
TS27: cerebellum Purkinje cell layer Weak 2L inset
Specimen 2M inset: postnatal day 4 (more )
Note: Double labeled: green - HNF6 (Onecut1); red - Calb1 (assay MGI:6490621);
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present 2M inset Expression in all Purkinje cells but not in other cerebellar cell types.
Specimen 2N inset: postnatal day 7 (more )
Note: Double labeled: green - HNF6 (Onecut1); red - Calb1 (assay MGI:6490621);
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Weak 2N inset
Specimen 2O inset: postnatal day 14 (more )
Note: Double labeled: green - HNF6 (Onecut1); red - Calb1 (assay MGI:6490621);
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Absent 2O inset
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory