Antibody:	anti-HMGN1
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS17: trunk surface ectoderm	Present		2Aa , S1A
TS17: neural tube	Strong	Widespread	2Aa , S1A	There is strong expression in all three layers--the outer marginal layer, the intermediate mantle layer, and the inner ependymal layer.
TS17: dorsal root ganglion	Present		2Aa , S1A
TS17: trunk mesenchyme	Present		2Aa , S1A

Structure	Level	Pattern	Image	Note
TS22: spinal cord roof plate	Present		S1C , 2Ab
TS22: spinal cord ventricular layer	Present		S1C , 2Ab	Expression is seen in the ependymal layer.
TS22: spinal cord mantle layer	Present	Non-Uniform	2Ad , S1C , S1G , 2Ab , S1E , 2Ac	Expresson is localized primarily to the dorsal half of the layer.
TS22: spinal cord marginal layer	Absent		2Ab , S1C

Structure	Level	Pattern	Image	Note
TS20: stomach epithelium	Present		S2A , 2Ba	Expression is strongest in the outermost mesothelium.
TS20: stomach mesenchyme	Present		S2A , 2Ba	Expression is strongest in the outermost mesothelium.

Structure	Level	Pattern	Image	Note
TS24: stomach epithelium	Present	Regionally restricted	2Bc , S2C , 2Bb , S2E	Expression is localized to the basal layer.
TS24: stomach muscularis externa	Present		S2G , 2Bd , S2C , 2Bb	Expression is localized to the longitudinal muscle layer.

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Present		2Ca , S3A	Expression is seen in bronchioles.
TS20: lung mesenchyme	Present		S3A , 2Ca

Structure	Level	Pattern	Image	Note
TS24: lung epithelium	Present	Non-Uniform	S3E , S3C , 2Cb , 2Cc	Expression is localized to the developing distal epithelium; it is down regulated in the proximal region.
TS24: bronchiole	Weak		2Cb , 2Cd , S3C , S3G	Expression is down regulated in the bronchiole.