Gene Expression Data
Assay Details
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Assay
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Reference:
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J:176585
Zheng Z, et al., Developmental basis of sexually dimorphic digit ratios. Proc Natl Acad Sci U S A. 2011 Sep 27;108(39):16289-94
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6490444
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Gene symbol:
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Esr1
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Gene name:
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estrogen receptor 1 (alpha)
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Results
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Specimen
2I:
embryonic day 12.5
(more  )
Specimen
2I:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Male
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb digit 2 |
Present |
Widespread |
2I
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Expression is present in ~85% of the cells, but only 10% of the cells show nuclear localization. The 4th digit has higher levels of expression than the second digit. Expression levels and type of expression are comparable between males and females.
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| TS20: hindlimb digit 4 |
Present |
Widespread |
2I
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Expression is present in ~85% of the cells, but only 10% of the cells show nuclear localization. The 4th digit has higher levels of expression than the second digit. Expression levels and type of expression are comparable between males and females.
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Specimen
2J:
embryonic day 12.5
(more )
Specimen
2J:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Female
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb digit 2 |
Present |
Widespread |
2J
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Expression is present in ~85% of the cells, but only 10% of the cells show nuclear localization. The 4th digit has higher levels of expression than the second digit. Expression levels and type of expression are comparable between males and females.
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| TS20: hindlimb digit 4 |
Present |
Widespread |
2J
,
2K
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Expression is present in ~85% of the cells, but only 10% of the cells show nuclear localization. The 4th digit has higher levels of expression than the second digit. Expression levels and type of expression are comparable between males and females.
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Specimen
2M:
embryonic day 14.5
(more )
Specimen
2M:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hindlimb digit 2 |
Present |
Widespread |
2M
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Expression is widespread; most of it is activated (nuclear). The 4th digit has higher levels of expression than the second digit. Expression levels in both digits are lower in males than females.
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| TS22: hindlimb digit 4 |
Present |
Widespread |
2M
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Expression is widespread; most of it is activated (nuclear). The 4th digit has higher levels of expression than the second digit. Expression levels in both digits are lower in males than females.
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Specimen
2N:
embryonic day 14.5
(more )
Specimen
2N:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Female
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hindlimb digit 2 |
Present |
Widespread |
2N
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Expression is widespread; most of it is activated (nuclear). The 4th digit has higher levels of expression than the second digit. Expression levels in both digits are higher in females than males.
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| TS22: hindlimb digit 4 |
Present |
Widespread |
2O
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2N
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Expression is widespread; most of it is activated (nuclear). The 4th digit has higher levels of expression than the second digit. Expression levels in both digits are higher in females than males.
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Specimen
S1D left:
embryonic day 16.5
(more )
Note: longitudinal section; proximal phalange
Specimen
S1D left:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Sex:
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Male
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Type:
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section
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Note:
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longitudinal section; proximal phalange
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hindlimb digit 2 |
Present |
Regionally restricted |
S1D left
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Expression is seen in the proximal phalange, especially in the hypertrophic zone; most is nuclear. There is no longer a significant difference in expression levels between the 2nd and 4th digit.
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Specimen
S1D right:
embryonic day 16.5
(more )
Note: longitudinal section; proximal phalange
Specimen
S1D right:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Sex:
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Male
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Type:
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section
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Note:
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longitudinal section; proximal phalange
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hindlimb digit 4 |
Present |
Regionally restricted |
S1D right
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Expression is seen in the proximal phalange, especially in the hypertrophic zone; most is nuclear. There is no longer a significant difference in expression levels between the 2nd and 4th digit.
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Specimen
S1E left:
embryonic day 16.5
(more )
Note: longitudinal section; proximal phalange
Specimen
S1E left:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Sex:
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Female
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Type:
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section
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Note:
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longitudinal section; proximal phalange
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hindlimb digit 2 |
Present |
Regionally restricted |
S1E left
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Expression is seen in the proximal phalange, especially in the hypertrophic zone; most is nuclear. There is no longer a significant difference in expression levels between the 2nd and 4th digit.
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Specimen
S1E right:
embryonic day 16.5
(more )
Note: longitudinal section; proximal phalange
Specimen
S1E right:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Sex:
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Female
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Type:
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section
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Note:
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longitudinal section; proximal phalange
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hindlimb digit 4 |
Present |
Regionally restricted |
S1E right
,
S1F
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Expression is seen in the proximal phalange, especially in the hypertrophic zone; most is nuclear. There is no longer a significant difference in expression levels between the 2nd and 4th digit.
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