Probe:	MGC clone 38046
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present	Widespread	2A	Expression in whole limb buds. Expression in broad region.
TS15: hindlimb ridge	Present	Widespread	2A	Expression in whole limb buds. Expression in broad region.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present	Regionally restricted	2B	Expression in areas between cartilage primordia.
TS20: hindlimb mesenchyme	Present	Regionally restricted	2B	Expression in areas between cartilage primordia.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	2C	Expression down-regulated in proximal region where mesenchymal cells condense and differentiate into chondrocytes. No expression in chondrocyte-lineage cells.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	2D	Expression down-regulated in proximal region where mesenchymal cells condense and differentiate into chondrocytes. No expression in chondrocyte-lineage cells.
TS19: trunk mesenchyme	Present	Regionally restricted	2D	Expression in cells between condensations that form future ribs.

Structure	Level	Pattern	Image	Note
TS20: hindlimb interdigital region mesenchyme	Strong		2E	Expression most abundant in interdigital mesenchyme.
TS20: hindlimb digit mesenchyme	Absent		2E	No expression in chondrocyte-lineage cells.

Structure	Level	Pattern	Image	Note
TS21: forelimb interdigital region mesenchyme	Strong		2F	Expression most abundant in interdigital mesenchyme.
TS21: forelimb digit mesenchyme	Absent		2F	No expression in chondrocyte-lineage cells.

Structure	Level	Pattern	Image	Note
TS20: mandibular process	Present	Regionally restricted	2K	Expression in osteogenic mesenchyme surrounding Meckel's cartilage.
TS20: Meckel's cartilage	Absent		2K	No expression in chondrocyte-lineage cells.

Structure	Level	Pattern	Image	Note
TS20: mandibular process	Present	Regionally restricted	2L	Expression in osteogenic mesenchyme surrounding Meckel's cartilage.
TS20: Meckel's cartilage	Absent		2L	No expression in chondrocyte-lineage cells.

Structure	Level	Pattern	Image	Note
TS20: mandibular process	Present	Regionally restricted	2M	Expression in osteogenic mesenchyme surrounding Meckel's cartilage.
TS20: Meckel's cartilage	Absent		2M	No expression in chondrocyte-lineage cells.

Structure	Level	Pattern	Image	Note
TS22: upper arm rest of mesenchyme	Present	Regionally restricted	2Q	Expression in perichondrium of hypertrophic chondrocyte region
TS22: humerus cartilage condensation	Absent		2Q	Expression absent from more centrally located chondrocytic cells.

Structure	Level	Pattern	Image	Note
TS23: humerus	Present	Regionally restricted	2S	Expression in prospective bone collar region and in developing bone trabeculae.

Structure	Level	Pattern	Image	Note
TS25: tibia	Present	Regionally restricted	2W	Expression overlaps with ocn but not with collagen 10.