Probe:	IMAGE clone 335997
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	3A	Expression was detected in the caudal part of the diencephalic anlage corresponding to the ventral area of prosomeres 1 and 2.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	3B	The rostral diencephalic domain extends: (i) anteriorly, into the basal plate of prosomeres 3 and 4 (p3,p4), anlage of the mammillary area; (ii) dorsally, along the p2-p3 boundary corresponding to the zona limitans (ZL), which will separate the dorsal from the ventral thalamus; and (iii) posteriorly to the pretectum (p1). The mammillary area corresponds to the only region where Barhl1 is expressed in the ventral midline. In the pretectum, two distinct domains of expression can be recognized, one in the basal plate and the other in the most alar and caudal region.
TS17: hindbrain	Present		3B	Expression was detected as a longitudinal stripe along the dorsal area of the alar plate, but not in the roof plate.
TS17: midbrain-hindbrain junction	Present		3B	Expression was detected as a longitudinal stripe along the dorsal area of the alar plate, but not in the roof plate.
TS17: future spinal cord	Present		3B	Expression was detected as a longitudinal stripe along the dorsal area of the alar plate, but not in the roof plate. Expression extended for the entire length of the spinal cord.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	3C	In the commissural area of the pretectum, expression extends basally and a positive band appears in the mesencephalic basal plate corresponding to the ventral periaqueductal grey.

Structure	Level	Pattern	Image	Note
TS20: mammillary area	Present		3D
TS20: diencephalon	Present	Regionally restricted	3D	Expression was detected in prerubral tegmentum, encompassing retromammillar (basal p3), posterior tubercle (basal p2) and subpretectal (basal p1) tegmental zones.
TS20: midbrain	Present	Regionally restricted	3D	Expression was detected in the rostral alar plate in the presumptive area of the superior colliculus.
TS20: hindbrain	Present	Regionally restricted	3D	Expression was detected in a large lateral band in the caudal rhombencephalon.
TS20: spinal cord mantle layer	Present	Regionally restricted	3D	Expression was confined to the mantle layer in a lateral band between the basal and alar plate.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	3E	The rostral diencephalic domain extends: (i) anteriorly, into the basal plate of prosomeres 3 and 4 (p3,p4), anlage of the mammillary area; (ii) dorsally, along the p2-p3 boundary corresponding to the zona limitans (ZL), which will separate the dorsal from the ventral thalamus; and (iii) posteriorly to the pretectum (p1). The mammillary area corresponds to the only region where Barhl1 is expressed in the ventral midline.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	3F	The rostral diencephalic domain extends: (i) anteriorly, into the basal plate of prosomeres 3 and 4 (p3,p4), anlage of the mammillary area; (ii) dorsally, along the p2-p3 boundary corresponding to the zona limitans (ZL), which will separate the dorsal from the ventral thalamus; and (iii) posteriorly to the pretectum (p1).

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	3G	The rostral diencephalic domain extends: (i) anteriorly, into the basal plate of prosomeres 3 and 4 (p3,p4), anlage of the mammillary area; (ii) dorsally, along the p2-p3 boundary corresponding to the zona limitans (ZL), which will separate the dorsal from the ventral thalamus; and (iii) posteriorly to the pretectum (p1).

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present	Regionally restricted	3H	Expression was detected as a longitudinal stripe along the dorsal area of the alar plate, but not in the roof plate.

Structure	Level	Pattern	Image	Note
TS20: mammillary area	Present		3I
TS20: diencephalon	Present	Regionally restricted	3I	Expression was detected in prerubral tegmentum, encompassing retromammillar (basal p3), posterior tubercle (basal p2) and subpretectal (basal p1) tegmental zones.

Structure	Level	Pattern	Image	Note
TS20: mammillary area	Present		3J
TS20: diencephalon	Present	Regionally restricted	3J	Expression was detected in prerubral tegmentum, encompassing retromammillar (basal p3), posterior tubercle (basal p2) and subpretectal (basal p1) tegmental zones.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	3K	In the commissural area of the pretectum, expression extends basally and a positive band appears in the mesencephalic basal plate corresponding to the ventral periaqueductal grey.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	3L	In the commissural area of the pretectum, expression extends basally and a positive band appears in the mesencephalic basal plate corresponding to the ventral periaqueductal grey.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	3M	The rostral diencephalic domain extends: (i) anteriorly, into the basal plate of prosomeres 3 and 4 (p3,p4), anlage of the mammillary area; (ii) dorsally, along the p2-p3 boundary corresponding to the zona limitans (ZL), which will separate the dorsal from the ventral thalamus; and (iii) posteriorly to the pretectum (p1). In the pretectum, two distinct domains of expression can be recognized, one in the basal plate and the other in the most alar and caudal region.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	3N	The basal expression in prosomeres 2 and 3 extends dorsally into the Zona limitans between the anlage of the ventral and dorsal thalamus.
TS19: midbrain	Present	Regionally restricted	3N	In the commissural area of the pretectum, expression extends basally and a positive band appears in the mesencephalic basal plate corresponding to the ventral periaqueductal grey.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	4A	Expression was detected as a longitudinal stripe along the dorsal area of the alar plate.
TS17: midbrain-hindbrain junction	Present	Regionally restricted	4A	Expression was detected as a longitudinal stripe along the dorsal area of the alar plate.
TS17: future spinal cord	Present	Regionally restricted	4A	Expression was detected as a longitudinal stripe along the dorsal area of the alar plate. Expression extended for the entire length of the spinal cord.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	4B	The expression domain in the caudal rhombencephalon and in spinal cord expands laterally following the ventral migrating cells from the dorsal alar plate.
TS19: hindbrain	Present	Regionally restricted	4B	The expression domain in the caudal rhombencephalon and in spinal cord expands laterally following the ventral migrating cells from the dorsal alar plate.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	4C	Expression was detected in the rostral alar plate in the presumptive area of the superior colliculus.
TS20: hindbrain	Present	Regionally restricted	4C	Expression was detected in a large lateral band in the caudal rhombencephalon.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	4D	Expression was detected as a longitudinal stripe along the dorsal area of the alar plate.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	4E	The expression domain in the spinal cord expands laterally following the ventral migrating cells from the dorsal alar plate.

Structure	Level	Pattern	Image	Note
TS20: spinal cord mantle layer	Present	Regionally restricted	4F	Expression was detected in two columns in the mantle zone between the basal and alar plates.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	4G	Expression was detected as a longitudinal stripe along the dorsal area of the alar plate.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	4H	The expression domain in the spinal cord expands laterally following the ventral migrating cells from the dorsal alar plate.

Structure	Level	Pattern	Image	Note
TS20: spinal cord mantle layer	Present	Regionally restricted	4I	Expression was detected in two columns in the mantle zone between the basal and alar plates.

Structure	Level	Pattern	Image	Note
TS24: inferior colliculus	Present	Graded	5A	Expression was detected in the caudal colliculus anlage with a distinct caudo-rostral gradient.
TS24: cerebellum external granule cell layer	Present		5A

Structure	Level	Pattern	Image	Note
TS24: brainstem	Present	Regionally restricted	5B	Expression was detected in the pontine nucleus.

Structure	Level	Pattern	Image	Note
TS24: brainstem	Present	Regionally restricted	5C	Expression was detected in the pontine nucleus.
TS24: inner ear epithelium	Present	Regionally restricted	5C	Expression was detected in the sensory epithelium of the inner ear.

Structure	Level	Pattern	Image	Note
TS28: inferior colliculus	Present		5D
TS28: superior colliculus	Present		5D
TS28: cerebellum external granule cell layer	Present	Regionally restricted	5D	Expression was detected in the most external part of the external granule cell layer.
TS28: pontine nucleus	Present		5D
TS28: mammillary body	Present	Regionally restricted	5D	Expression was detected in the mammillary nuclei.

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present	Regionally restricted	5E	Expression was detected in the most external part of the external granule cell layer.