Gene Expression Data
Assay Details
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Assay
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Reference:
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J:231660
Punzo D, et al., Age-Related Changes in d-Aspartate Oxidase Promoter Methylation Control Extracellular d-Aspartate Levels and Prevent Precocious Cell Death during Brain Aging. J Neurosci. 2016 Mar 9;36(10):3064-78
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6478316
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Gene symbol:
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Aif1
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Gene name:
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allograft inflammatory factor 1
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Antibody:
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anti-Iba-1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
5g 10 months +/+:
postnatal month 10
(more  )
Specimen
5g 10 months +/+:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal month 10
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5g 10 months -/-:
(close )
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Genetic Background:
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B6.129-Ddotm1Rdl
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Mutant Allele(s):
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Ddotm1Rdl/Ddotm1Rdl
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Age:
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postnatal month 10
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5g 13 months +/+:
postnatal month 13
(more )
Specimen
5g 13 months +/+:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal month 13
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5g 13 months -/-:
(close )
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Genetic Background:
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B6.129-Ddotm1Rdl
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Mutant Allele(s):
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Ddotm1Rdl/Ddotm1Rdl
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Age:
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postnatal month 13
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5g 10 months +/+ right:
postnatal month 10
(more )
Specimen
5g 10 months +/+ right:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal month 10
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5g 10 months -/- right:
(close )
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Genetic Background:
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B6.129-Ddotm1Rdl
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Mutant Allele(s):
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Ddotm1Rdl/Ddotm1Rdl
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Age:
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postnatal month 10
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5g 13 months +/+ right:
postnatal month 13
(more )
Specimen
5g 13 months +/+ right:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal month 13
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5g 13 months -/- right:
(close )
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Genetic Background:
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B6.129-Ddotm1Rdl
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Mutant Allele(s):
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Ddotm1Rdl/Ddotm1Rdl
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Age:
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postnatal month 13
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5g Resting:
(close )
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Genetic Background:
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B6.129-Ddotm1Rdl
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Mutant Allele(s):
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Ddotm1Rdl/Ddotm1Rdl
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Age:
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postnatal month 13
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5g Reactive:
(close )
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Genetic Background:
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B6.129-Ddotm1Rdl
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Mutant Allele(s):
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Ddotm1Rdl/Ddotm1Rdl
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Age:
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postnatal month 13
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5g Dystrophic:
(close )
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Genetic Background:
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B6.129-Ddotm1Rdl
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Mutant Allele(s):
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Ddotm1Rdl/Ddotm1Rdl
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Age:
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postnatal month 13
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: substantia nigra |
Present |
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5g Dystrophic
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The number of positive dystrophic microglia has increased in this mutant compared to age-matched controls.
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Analysis Tools