Gene Expression Data
Assay Details
Assay
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Reference:
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J:152476
Gunnersen JM, et al., Seizure-related gene 6 (Sez-6) in amacrine cells of the rodent retina and the consequence of gene deletion. PLoS One. 2009;4(8):e6546
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6478297
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Gene symbol:
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Sez6
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Gene name:
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seizure related gene 6
|
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Results
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Specimen
2A/B:
postnatal adult
(more )
Specimen
2A/B:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina outer plexiform layer |
Present |
|
2B
,
2A
|
Bipolar cell apical dentrites were positively stained.
|
TS28: retina inner nuclear layer |
Present |
Non-Uniform |
2A
,
2B
|
Expression was particularly prominent in the amacrine cells of the inner nuclear layer. Strongest immunoreactivity was observed in two rows of cells adjacent to the inner plexiform layer.
|
TS28: retina ganglion cell layer |
Present |
Non-Uniform |
2B
,
2A
|
Immunostained cells were observed in the ganglion cell layer (many of which are likely to be displaced amacrines). Immunreactivity was observed in bright puncta, frequently positioned adjacent to the nucleus and the apical dendrite.
|
TS28: retina inner plexiform layer |
Present |
Non-Uniform |
2A
,
2B
|
Fine puntate staining was observed extending into the inner plexiform layer along apical dendrites of the labeled neurons in the ganglion cell layer.
|
Specimen
2C/D:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Sez6tm1.1Sest/Sez6tm1.1Sest
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina |
Absent |
|
2D
,
2C
|
Low level diffuse non-specific staining was observed in ganglion cells, the inner and outer plexiform layers and photoreceptor outer segments.
|
Specimen
2E:
postnatal adult
(more )
Specimen
2E:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina inner nuclear layer |
Present |
Non-Uniform |
2E
|
Strongest immunoreactivity was observed in two rows of cells adjacent to the inner plexiform layer. Signal was concentrated strongly within a discrete perinuclear bright spot. More diffuse cytoplasmic staining was also observed in amacrine cells. Staining was noted in bipolar cell and horizontal cell somata in the outer part of the inner nuclear layer.
|
TS28: retina outer plexiform layer |
Present |
|
2E
|
Bipolar cell apical dentrites were positively stained.
|
Specimen
2F:
postnatal adult
(more )
Specimen
2F:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina inner nuclear layer |
Present |
Non-Uniform |
2F
|
Expression was noted in a horizontal cell. Strongest immunoreactivity was observed in two rows of cells adjacent to the inner plexiform layer.
|
TS28: retina ganglion cell layer |
Present |
Non-Uniform |
2F
|
Expression was detected in an alpha or A1 ganglion cell.
|
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