Probe:	Nkx2-3 genomic1
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum endoderm	Present	Regionally restricted	2A	Expression is in the ventral endoderm of the foregut pocket.

Structure	Level	Pattern	Image	Note
TS14: branchial arch	Present		2C
TS14: embryo mesoderm	Present	Regionally restricted	2C	Expression is in the visceral mesoderm.

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present		2D	Expression has a defined boundary inside the lip of the foregut pocket.
TS13: branchial arch endoderm	Present	Regionally restricted	2D	Expression is in the ventral pharyngeal endoderm.
TS13: buccopharyngeal membrane endoderm	Present		2D

Structure	Level	Pattern	Image	Note
TS13: branchial arch endoderm	Present	Regionally restricted	2E	Expression is in the pharyngeal floor endoderm.
TS13: branchial arch ectoderm	Present		2E
TS13: branchial pouch	Present		2E
TS13: oral region ectoderm	Present	Regionally restricted	2E	Expression is in the ectoderm of the oral floor.
TS13: foregut diverticulum	Present	Regionally restricted	2E	Expression throughout the full medio-lateral extent of the ventral surface of the pharynx.
TS13: 2nd branchial arch ectoderm	Weak		2E

Structure	Level	Pattern	Image	Note
TS13: branchial arch endoderm	Present	Regionally restricted	2F	Expression is in the pharyngeal floor endoderm.
TS13: branchial arch ectoderm	Present		2F
TS13: branchial pouch	Present		2F
TS13: oral region ectoderm	Present	Regionally restricted	2F	Expression is in the ectoderm of the oral floor.
TS13: foregut diverticulum	Present	Regionally restricted	2F	Expression throughout the full medio-lateral extent of the ventral surface of the pharynx.
TS13: 2nd branchial arch ectoderm	Weak		2F

Structure	Level	Pattern	Image	Note
TS13: branchial arch endoderm	Present	Regionally restricted	2G	Expression is in the pharyngeal floor endoderm.
TS13: branchial arch ectoderm	Present		2G
TS13: branchial pouch	Present		2G
TS13: oral region ectoderm	Present	Regionally restricted	2G	Expression is in the ectoderm of the oral floor that extends laterally into ectoderm associated with the cranial and dorsal part of the first pair of branchial arches.
TS13: foregut diverticulum	Present	Regionally restricted	2G	Expression throughout the full medio-lateral extent of the ventral surface of the pharynx.

Structure	Level	Pattern	Image	Note
TS13: branchial arch endoderm	Present	Regionally restricted	2H	Expression is in the pharyngeal floor endoderm.
TS13: branchial arch ectoderm	Present		2H
TS13: branchial pouch	Present		2H
TS13: buccopharyngeal membrane	Present		2H
TS13: oral region ectoderm	Present	Regionally restricted	2H	Expression is in the ectoderm of the oral floor that extends laterally into ectoderm associated with the cranial and dorsal part of the first pair of branchial arches.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch ectoderm	Present		2I
TS14: branchial pouch	Present	Regionally restricted	2I	Expression is in the foregut floor and extends laterally into the pouches.
TS14: foregut	Present	Regionally restricted	2I	Expression is in the foregut floor and extends laterally into the pouches.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch ectoderm	Present		2J
TS14: foregut	Present	Regionally restricted	2J	Expression is in the foregut floor and extends cranially into the oral floor.
TS14: oral region	Present	Regionally restricted	2J	Expression is in the foregut floor and extends cranially into the oral floor.

Structure	Level	Pattern	Image	Note
TS14: branchial arch endoderm	Present	Regionally restricted	2K	Expression is in the pharyngeal floor endoderm up to the level of the buccopharyngeal membrane and oral floor ectoderm.
TS14: foregut	Present	Regionally restricted	2K	Expression is in the foregut floor and extends caudally to approximately the level of the future lung bud.