Ret In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:294660 Vaquero M, et al., Sprouty1 Controls Genitourinary Development via its N-Terminal Tyrosine. J Am Soc Nephrol. 2019 Aug;30(8):1398-1411
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:6476733
Gene symbol:	Ret
Gene name:	ret proto-oncogene

Reporter:	GFP
	Direct Detection

Results

Specimen 6B E10.5 +/+: embryonic day 10.5; Ret^tm13.1Jmi/Ret⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: ureteric bud	Present		6B E10.5 +/+

Specimen 6B E10.5 Y53ANeo/Y53ANeo: embryonic day 10.5; Ret^tm13.1Jmi/Ret⁺, Spry1^tm1Mns/Spry1^tm1Mns (more

)

Structure	Level	Pattern	Image	Note
TS17: ureteric bud	Present		6B E10.5 Y53ANeo/Y53ANeo	The ureteric bud forms normally in the mutant.
TS17: nephric duct, metanephric portion	Present		6B E10.5 Y53ANeo/Y53ANeo	Protuberances of the Wolffian duct cranial to the ureteric bud are observed in the mutant but not the wild type.

Specimen 6B E10.5 -/-: embryonic day 10.5; Ret^tm13.1Jmi/Ret⁺, Spry1^tm1.1Jdli/Spry1^tm1.1Jdli (more

)

Structure	Level	Pattern	Image	Note
TS17: ureteric bud	Present		6B E10.5 -/-	The ureteric bud forms normally in the mutant.
TS17: nephric duct, metanephric portion	Present		6B E10.5 -/-	Protuberances of the Wolffian duct cranial to the ureteric bud are observed in the mutant but not the wild type.

Specimen 6B E11.5 +/+: embryonic day 11.5; Ret^tm13.1Jmi/Ret⁺ (more

)

Structure	Level	Image	Note
TS19: ureteric bud	Present	6B E11.5 +/+
TS19: nephric duct, metanephric portion	Present	6B E11.5 +/+	Expression is seen in the Wolffian duct.
TS19: common nephric duct	Present	6B E11.5 +/+

Specimen 6B E11.5 Y53ANeo/Y53ANeo: embryonic day 11.5; Ret^tm13.1Jmi/Ret⁺, Spry1^tm1Mns/Spry1^tm1Mns (more

)

Structure	Level	Image	Note
TS19: ureteric bud	Present	6B E11.5 Y53ANeo/Y53ANeo	The stalk of the main ureteric bud is shorter than that seen in wild type; numerous ectopic buds are also seen.
TS19: nephric duct, metanephric portion	Present	6B E11.5 Y53ANeo/Y53ANeo	Expression is seen in the Wolffian duct.
TS19: common nephric duct	Present	6B E11.5 Y53ANeo/Y53ANeo

Specimen 6B E11.5 -/-: embryonic day 11.5; Ret^tm13.1Jmi/Ret⁺, Spry1^tm1.1Jdli/Spry1^tm1.1Jdli (more

)

Structure	Level	Image	Note
TS19: ureteric bud	Present	6B E11.5 -/-	The stalk of the main ureteric bud is shorter than that seen in wild type; numerous ectopic buds are also seen.
TS19: nephric duct, metanephric portion	Present	6B E11.5 -/-	Expression is seen in the Wolffian duct.
TS19: common nephric duct	Present	6B E11.5 -/-

Specimen 6B E12.5 +/+: embryonic day 12.5; Ret^tm13.1Jmi/Ret⁺ (more

)

Structure	Level	Image	Note
TS20: ureter	Present	6B E12.5 +/+	In wild type the ureter is well-separated from the Wolffian duct.
TS20: nephric duct, metanephric portion	Present	6B E12.5 +/+	Expression is seen in the Wolffian duct.
TS20: common nephric duct	Present	6B E12.5 +/+	In wild type the cnd is short.

Specimen 6B E12.5 Y53ANeo/Y53ANeo: embryonic day 12.5; Ret^tm13.1Jmi/Ret⁺, Spry1^tm1Mns/Spry1^tm1Mns (more

)

Structure	Level	Image	Note
TS20: ureter	Present	6B E12.5 Y53ANeo/Y53ANeo	In the mutant the ureter is not well-separated from the Wolffian duct. In addition, shorter, supernumary ectopic ureteric buds are observed.
TS20: nephric duct, metanephric portion	Present	6B E12.5 Y53ANeo/Y53ANeo	Expression is seen in the Wolffian duct.
TS20: common nephric duct	Present	6B E12.5 Y53ANeo/Y53ANeo	In the mutant the cnd is longer than in wild type.

Specimen 6B E12.5 -/-: embryonic day 12.5; Ret^tm13.1Jmi/Ret⁺, Spry1^tm1.1Jdli/Spry1^tm1.1Jdli (more

)

Structure	Level	Image	Note
TS20: primitive collecting duct	Present	6B E12.5 -/-	In the mutant the ureter is not well-separated from the Wolffian duct. In addition, shorter, supernumary ectopic ureteric buds are observed.
TS20: nephric duct, metanephric portion	Present	6B E12.5 -/-	Expression is seen in the Wolffian duct.
TS20: common nephric duct	Present	6B E12.5 -/-	In the mutant the cnd is longer than in wild type.

Specimen 6B E14.5 +/+: embryonic day 14.5; Ret^tm13.1Jmi/Ret⁺ (more

)

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present		6B E14.5 +/+

Specimen 6B E14.5 Y53ANeo/Y53ANeo: embryonic day 14.5; Ret^tm13.1Jmi/Ret⁺, Spry1^tm1Mns/Spry1^tm1Mns (more

)

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present		6B E14.5 Y53ANeo/Y53ANeo

Specimen 6B E14.5 -/-: embryonic day 14.5; Ret^tm13.1Jmi/Ret⁺, Spry1^tm1.1Jdli/Spry1^tm1.1Jdli (more

)

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present		6B E14.5 -/-

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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