Sulf1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:169510 Buresh RA, et al., Sulfatase 1 is an inhibitor of ductal morphogenesis with sexually dimorphic expression in the urogenital sinus. Endocrinology. 2010 Jul;151(7):3420-31
Assay type:	RT-PCR
MGI Accession ID:	MGI:6473893
Gene symbol:	Sulf1
Gene name:	sulfatase 1

Probe:	Sulf1-pE, Sulf1-pF
Probe preparation:	labelled with SYBR green
Assay notes:	Real-time RT-PCR was used to obtain these results. Expression values were normalized to 18S mRNA.

Results

Image: 1A

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
e16 - female	E16.0 (a)	TS24: pelvic urethra of female	Present	2 µg; total RNA	CD-1		Female
e16 - male	E16.0 (a)	TS24: pelvic urethra of male	Present (b)	2 µg; total RNA	CD-1		Male
e17 - female	E17.0 (a)	TS25: pelvic urethra of female	Present	2 µg; total RNA	CD-1		Female
e17 - male	E17.0 (a)	TS25: pelvic urethra of male	Present (c)	2 µg; total RNA	CD-1		Male
e18 - female	E18.0 (a)	TS26: pelvic urethra of female	Present	2 µg; total RNA	CD-1		Female
e18 - male	E18.0 (a)	TS26: pelvic urethra of male	Weak (d)	2 µg; total RNA	CD-1		Male

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression levels are comparable between males and females at E16.
(c) Expression levels are comparable between males and females at E17.
(d) Expression levels are dramatically down-regulated in the male relative to the female at E18.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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