Probe:	Runx2 probe17
Probe preparation:	Antisense labelled with P32 RNA

Structure	Level	Pattern	Image	Note
TS20: mandibular process mesenchyme	Strong	Regionally restricted	S1B	Intense expression in mesenchymal condensates of forming bones and teeth in developing maxillary and mandibular arches.
TS20: maxillary process mesenchyme	Strong	Regionally restricted	S1B	Intense expression in mesenchymal condensates of forming bones and teeth in developing maxillary and mandibular arches.

Structure	Level	Pattern	Image	Note
TS22: jaw mesenchyme	Strong	Regionally restricted	1B	Highly expressed in mesenchymal cells in alveolar bone.
TS22: dental papilla	Strong		1B	Highly expressed in dental papilla and dental follicle.
TS22: dental sac	Strong		1B	Highly expressed in dental papilla and dental follicle.
TS22: tooth epithelium	Trace		1B	Expression barely seen in dental epithelium.

Structure	Level	Pattern	Image	Note
TS24: jaw mesenchyme	Present	Regionally restricted	1F	Expression in differentiating osteogenic mesenchyme.
TS24: enamel epithelium	Present	Regionally restricted	1F	Expression in ameloblasts.
TS24: tooth mesenchyme	Present	Regionally restricted	1F	Expression in dental pulp cells.

Structure	Level	Pattern	Image	Note
TS26: lower jaw incisor	Present	Regionally restricted	1J	Expression down-regulated in odontoblast, ameloblast and dental pulp cells of mandibular incisor and first molar.
TS26: lower jaw molar	Present	Regionally restricted	1J	Expression down-regulated in odontoblast, ameloblast and dental pulp cells of mandibular incisor and first molar.
TS26: enamel epithelium	Weak	Regionally restricted	1J	Expression dramatically down-regulated in ameloblasts.
TS26: tooth mesenchyme	Weak	Regionally restricted	1J	Expression dramatically down-regulated in odontoblasts, and dental pulp cells, except for cells near mesenchyme within alveolar bone in developing incisor and molar.
TS26: jaw mesenchyme	Present	Regionally restricted	1J	Expression clearly detected in osteogenic mesenchyme. Expression in differentiating bone osteoblasts.

Structure	Level	Pattern	Image	Note
TS26: enamel epithelium	Weak	Regionally restricted	S1F	Expression dramatically down-regulated in ameloblasts.
TS26: tooth mesenchyme	Weak	Regionally restricted	S1F	Expression dramatically down-regulated in odontoblasts, and dental pulp cells, except for cells near mesenchyme within alveolar bone in developing incisor and molar.
TS26: jaw mesenchyme	Present	Regionally restricted	S1F	Expression clearly detected in osteogenic mesenchyme. Expression in differentiating bone osteoblasts.

Structure	Level	Pattern	Image	Note
TS26: enamel epithelium	Weak	Regionally restricted	S1J	Expression dramatically down-regulated in ameloblasts.
TS26: tooth mesenchyme	Weak	Regionally restricted	S1J	Expression dramatically down-regulated in odontoblasts, and dental pulp cells, except for cells near mesenchyme within alveolar bone in developing incisor and molar.
TS26: jaw mesenchyme	Present	Regionally restricted	S1J	Expression clearly detected in osteogenic mesenchyme. Expression in differentiating bone osteoblasts.

Structure	Level	Pattern	Image	Note
TS27: jaw skeleton	Strong	Regionally restricted	2B	Expression in osteogenic mesenchyme. Highly expressed in osteoblasts.
TS27: incisor dental pulp	Weak	Regionally restricted	2B	Weak expression in odontoblasts and dental pulp cells in developing incisor and molar.
TS27: molar dental pulp	Weak	Regionally restricted	2B	Weak expression in odontoblasts and dental pulp cells in developing incisor and molar.

Structure	Level	Pattern	Image	Note
TS27: jaw skeleton	Strong	Regionally restricted	S2B	Expression in osteogenic mesenchyme. Highly expressed in osteoblasts.
TS27: incisor dental pulp	Weak	Regionally restricted	S2B	Weak expression in odontoblasts and dental pulp cells in developing incisor and molar.
TS27: molar dental pulp	Weak	Regionally restricted	S2B	Weak expression in odontoblasts and dental pulp cells in developing incisor and molar.

Structure	Level	Pattern	Image	Note
TS28: jaw skeleton	Strong	Regionally restricted	2F	Similar expression patterns to those seen at PN1. Highly expressed in osteoblasts.
TS28: incisor dental pulp	Weak	Regionally restricted	2F	Weak expression in odontoblasts and dental pulp cells in developing incisor and molar.
TS28: molar dental pulp	Weak	Regionally restricted	2F	Weak expression in odontoblasts and dental pulp cells in developing incisor and molar.

Structure	Level	Pattern	Image	Note
TS28: alveolar process	Present	Regionally restricted	S2F	Expression in alveolar bone osteoblasts
TS28: tooth substance	Present	Regionally restricted	S2F	Expression in cells within cemento-enamel junction and roots.
TS28: tooth root	Present		S2F
TS28: molar dental pulp	Weak	Regionally restricted	S2F	Weak expression in odontoblasts and dental pulp cells in developing molars.

Structure	Level	Pattern	Image	Note
TS28: alveolar process	Present	Regionally restricted	2J	Expression in alveolar bone osteoblasts
TS28: tooth substance	Present	Regionally restricted	2J	Expression in cells within cemento-enamel junction and roots.
TS28: tooth root	Present		2J
TS28: molar dental pulp	Weak	Regionally restricted	2J	Weak expression in odontoblasts and dental pulp cells in developing molars.

Structure	Level	Pattern	Image	Note
TS28: alveolar process	Present	Regionally restricted	S2J	Expression in alveolar bone osteoblasts
TS28: tooth substance	Present	Regionally restricted	S2J	Expression in cells within cemento-enamel junction and roots.
TS28: tooth root	Present		S2J
TS28: molar dental pulp	Present	Regionally restricted	S2J	Weak expression in odontoblasts and dental pulp cells in developing molars.

Structure	Level	Pattern	Image	Note
TS28: molar dental pulp	Weak	Regionally restricted	S2N	Weak expression in odontoblasts and dental pulp cells in developing molars.

Structure	Level	Pattern	Image	Note
TS28: molar dental pulp	Weak	Regionally restricted	S2R	Weak expression in odontoblasts and dental pulp cells in developing molars.