Gene Expression Data
Assay Details
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Assay
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Reference:
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J:236232
Wang W, et al., Smad2 and Smad3 Regulate Chondrocyte Proliferation and Differentiation in the Growth Plate. PLoS Genet. 2016 Oct;12(10):e1006352
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6473215
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Gene symbol:
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Hdac4
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Gene name:
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histone deacetylase 4
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Antibody:
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Anti-Hdac4 (#2072)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
6A Smad2:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6J * CD-1 * SJL
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Mutant Allele(s):
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Smad2tm2Rob/Smad2tm2Rob
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Age:
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embryonic day 18.5
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Age Note:
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The text referred to this specimen's age as P0, while the figure legend indicated it was E18.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: tibia |
Present |
Regionally restricted |
6A Smad2fx/fx
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Expression was detected in the lower proliferative prehypertrophic and hypertrophic zones, and was localized in the nucleus.
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Specimen
6A Smad2:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J * CD-1 * SJL
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Mutant Allele(s):
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Smad2tm2Rob/Smad2tm2Rob
Tg(Col2a1-cre)1Bhr/0
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Age:
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embryonic day 18.5
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Age Note:
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The text referred to this specimen's age as P0, while the figure legend indicated it was E18.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: tibia |
Present |
Regionally restricted |
6A Smad2cko
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Expression was greatly diminished in the growth plate of the mutant compared with control.
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Specimen
6A Smad3<-/->:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J * CD-1 * SJL
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Mutant Allele(s):
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Smad3tm1Par/Smad3tm1Par
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Age:
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embryonic day 18.5
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Age Note:
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The text referred to this specimen's age as P0, while the figure legend indicated it was E18.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
6A Smad2;Smad3<-/->:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J * CD-1 * SJL
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Mutant Allele(s):
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Smad2tm2Rob/Smad2tm2Rob
Smad3tm1Par/Smad3tm1Par
Tg(Col2a1-cre)1Bhr/0
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Age:
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embryonic day 18.5
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Age Note:
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The text referred to this specimen's age as P0, while the figure legend indicated it was E18.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: tibia |
Present |
Regionally restricted |
6A Smad2cko;Smad3-/-
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Expression was greatly diminished in the growth plate of the double mutant compared with control. The percentage of cells expressing nuclear Hdac4 was significantly decreased at the border of the prehypertrophic and lower columnar zones of the double mutant compared to either Smad2<cko> or Smad3<-/->.
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)
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