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Gene Expression Data
Assay Details
Assay
Reference: J:236232 Wang W, et al., Smad2 and Smad3 Regulate Chondrocyte Proliferation and Differentiation in the Growth Plate. PLoS Genet. 2016 Oct;12(10):e1006352
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6473169
Gene symbol: Smad3
Gene name: SMAD family member 3
Antibody: phospho-Smad2 (Ser465/467) (138D4) mAb (#3108)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: The antibody recognized both Smad2 and Smad3 phosphorylated forms.
Results
Specimen S1A: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: tibia Present Regionally restricted S1A Expression was detected throughout the resting, columnar, and prehypertrophic zones of the proximal tibia growth plate.
Specimen S1B: embryonic day 16.5; Smad2tm2Rob/Smad2tm2Rob, Tg(Col2a1-cre)1Bhr/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: tibia Present Regionally restricted S1B Expression was detected throughout the resting, columnar, and prehypertrophic zones of the proximal tibia growth plate.
Specimen S1C: embryonic day 16.5; Smad3tm1Par/Smad3tm1Par (more )
Structure Level Pattern Image Note
TS24: tibia Absent S1C The apparent staining is due to the presence of Smad2.
Specimen S1D: embryonic day 16.5; Smad2tm2Rob/Smad2tm2Rob, Smad3tm1Par/Smad3tm1Par, Tg(Col2a1-cre)1Bhr/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: tibia Absent S1D
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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