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Gene Expression Data
Assay Details
Assay
Reference: J:173123 Mahoney Rogers AA, et al., Sprouty1 and Sprouty2 limit both the size of the otic placode and hindbrain Wnt8a by antagonizing FGF signaling. Dev Biol. 2011 May 1;353(1):94-104
Assay type: RNA in situ
MGI Accession ID: MGI:6471920
Gene symbol: Spry1
Gene name: sprouty RTK signaling antagonist 1
Probe: Spry1 probe7
Probe preparation: labelled with digoxigenin RNA
Results
Specimen 3A: embryonic day 8.0; Spry1tm1.1Jdli/Spry1+, Spry2tm1.1Mrt/Spry2+ (more )
Note: 5 somite stage.
Structure Level Pattern Image Note
TS12: otic pre-placode Present 3A Expression in ectoderm of pre-otic field.
TS12: head mesenchyme Present Regionally restricted 3A Expression in layer of mesenchymal cells just underlying the ectoderm.
TS12: branchial arch endoderm Present 3A Expression in pharyngeal endoderm and adjacent pharyngeal mesenchyme
TS12: branchial arch mesenchyme Present 3A Expression in pharyngeal endoderm and adjacent pharyngeal mesenchyme
TS12: embryo endoderm Present Regionally restricted 3A Expression in underlying ventral endoderm.
TS12: future hindbrain Absent 3A No expression in hindbrain adjacent to otic region.
Specimen 3B: embryonic day 8.5; Spry1tm1.1Jdli/Spry1+, Spry2tm1.1Mrt/Spry2+ (more )
Note: 8 somite stage.
Structure Level Pattern Image Note
TS13: otic placode epithelium Present 3B Expression in placodal ectoderm.
TS13: otic placode mesenchyme Absent 3B No expression in mesenchyme underlying otic placode.
TS13: head surface ectoderm Present Regionally restricted 3B Expression in surface ectoderm ventral to otic placode.
TS13: branchial arch endoderm Present 3B Expression in pharyngeal endoderm and adjacent pharyngeal mesenchyme
TS13: branchial arch mesenchyme Present 3B Expression in pharyngeal endoderm and adjacent pharyngeal mesenchyme
TS13: embryo endoderm Present Regionally restricted 3B Expression in underlying ventral endoderm.
TS13: future hindbrain Absent 3B No expression in hindbrain adjacent to otic region.
Specimen 3C: embryonic day 8.5; Spry1tm1.1Jdli/Spry1+, Spry2tm1.1Mrt/Spry2+ (more )
Note: 11 somite stage.
Structure Level Pattern Image Note
TS13: otic placode Present Widespread 3C Expression in all cells of otic placode.
TS13: otic placode mesenchyme Absent 3C No expression in underlying mesenchyme.
TS13: head surface ectoderm Ambiguous 3C No expression in surface ectoderm immediately ventral to otic placode.
TS13: branchial arch endoderm Present 3C Expression in pharyngeal endoderm and adjacent pharyngeal mesenchyme
TS13: branchial arch mesenchyme Present 3C Expression in pharyngeal endoderm and adjacent pharyngeal mesenchyme
TS13: embryo endoderm Present Regionally restricted 3C Expression in underlying ventral endoderm.
TS13: future hindbrain Absent 3C No expression in hindbrain adjacent to otic region.
Specimen 3D: embryonic day 9.0; Spry1tm1.1Jdli/Spry1+, Spry2tm1.1Mrt/Spry2+ (more )
Note: 13 somite stage.
Structure Level Pattern Image Note
TS14: otic placode Present Regionally restricted 3D Expression enriched in medial regions of otic placode, closer to hindbrain.
TS14: otic placode mesenchyme Absent 3D No expression in underlying mesenchyme.
TS14: head surface ectoderm Ambiguous Regionally restricted 3D No expression in surface ectoderm immediately ventral to otic placode.
TS14: branchial arch endoderm Present 3D Expression in pharyngeal endoderm and adjacent pharyngeal mesenchyme
TS14: branchial arch mesenchyme Present 3D Expression in pharyngeal endoderm and adjacent pharyngeal mesenchyme
TS14: future hindbrain Absent 3D No expression in hindbrain adjacent to otic region.
Specimen 3I: embryonic day 8.0; Spry1tm1.1Jdli/Spry1+, Spry2tm1.1Mrt/Spry2+ (more )
Note: 6 somite stage.
Structure Level Pattern Image Note
TS12: otic pre-placode Present 3I Broad domain of expression lateral to hindbrain.
Specimen 3K: embryonic day 8.0; Spry1tm1.1Jdli/Spry1+, Spry2tm1.1Mrt/Spry2+ (more )
Note: 4 somite stage.
Structure Level Pattern Image Note
TS12: otic pre-placode Present Regionally restricted 3K Expression in localized basal region of ectoderm cells, but not in apical region of these cells.
Specimen 3L: embryonic day 8.5; Spry1tm1.1Jdli/Spry1+, Spry2tm1.1Mrt/Spry2+ (more )
Note: 8 somite stage.
Structure Level Pattern Image Note
TS12: otic pre-placode Present Regionally restricted 3L Expression in localized basal region of ectoderm cells, but not in apical region of these cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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