Probe:	Sim1 cDNA6
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	4C	Expressed in the basal portion of the caudal diencephalon.
TS15: future midbrain	Present		4C
TS15: head mesenchyme	Present	Regionally restricted	4C	Expressed in the mesenchyme underlying the neural tube in the cephalic flexure.
TS15: dermomyotome	Strong		4C

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	4E	Expressed in the mammillary area in the basal half but excluded from the midline.
TS15: head mesenchyme	Present	Regionally restricted	4E	Expressed in the mesenchyme underlying the neural tube in the cephalic flexure.

Structure	Level	Pattern	Image	Note
TS17: diencephalon mantle layer	Present		5A
TS17: diencephalon ventricular layer	Present		5A
TS17: midbrain mantle layer	Present		5A
TS17: midbrain ventricular layer	Present		5A
TS17: diencephalon	Present	Regionally restricted	5A	Expressed in the hypothalamic domain. Dorsal extended expression at the zona limitans.
TS17: head mesenchyme	Present	Regionally restricted	5A	Expressed in a restricted region.
TS17: telencephalon	Present	Regionally restricted	5A	Expressed in the alar plate.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	5G , 5C	Dorsal extended expression at the zona limitans in a V-shaped group of cells that spaned the region between the ridge and the furrow.

Structure	Level	Pattern	Image	Note
TS17: diencephalon mantle layer	Present		5I
TS17: diencephalon ventricular layer	Present		5I
TS17: midbrain mantle layer	Present		5I
TS17: midbrain ventricular layer	Present		5I

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	5K	Expressed in the hypothalamic domain.
TS17: diencephalon mantle layer	Present		5K
TS17: diencephalon ventricular layer	Present		5K
TS17: midbrain mantle layer	Present		5K
TS17: midbrain ventricular layer	Present		5K
TS17: telencephalon	Present	Regionally restricted	5K	Expressed in the alar plate.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	5M	Expressed in a restricted region.
TS17: diencephalon	Present	Regionally restricted	5M	Expressed in the hypothalamic domain.
TS17: diencephalon mantle layer	Present		5M
TS17: diencephalon ventricular layer	Present		5M
TS17: midbrain mantle layer	Present		5M
TS17: midbrain ventricular layer	Present		5M

Structure	Level	Pattern	Image	Note
TS15: unsegmented mesenchyme	Present	Uniform	8A
TS15: nephric duct	Strong		8A

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	8B	Expression gradually excluded from the ventromedial half of the somite.

Structure	Level	Pattern	Image	Note
TS15: dermomyotome	Present		8C
TS15: sclerotome	Absent		8C
TS15: nephric duct	Strong		8C

Structure	Level	Pattern	Image	Note
TS17: body region	Present	Regionally restricted	8G	Expressed in the girdle.
TS17: future spinal cord	Present	Regionally restricted	8G	Expressed in two bands of cells flanking the floor plate.
TS17: forelimb bud	Weak	Regionally restricted	8G	Expressed in the dorsal aspect.

Structure	Level	Pattern	Image	Note
TS14: future diencephalon	Present	Regionally restricted		Expressed in the caudal diencephalon.

Structure	Level	Pattern	Image	Note
TS16: neural tube lateral wall	Present	Regionally restricted		Expressed in bilateral stripes flanking the floor plate.

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present	Regionally restricted		Expressed in the mantle.
TS17: midbrain	Present	Regionally restricted		Expressed in largely a longitudinal basal plate zone.

Structure	Level	Pattern	Image	Note
TS20: dermis	Present
TS20: diencephalon mantle layer	Present
TS20: diencephalon ventricular layer	Present
TS20: early nephron	Present
TS20: hypothalamus	Present
TS20: mammillary area	Present
TS20: midbrain mantle layer	Present
TS20: midbrain ventricular layer	Present
TS20: telencephalon	Present

Structure	Level	Pattern	Image	Note
TS24: mammillary area	Present	Regionally restricted		Expressed in the lateral mammillary nuclei.
TS24: amygdala	Present
TS24: anterior hypothalamic nucleus	Present
TS24: genital tubercle	Present
TS24: head mesenchyme	Present
TS24: maturing glomerular tuft	Present
TS24: renal tubule	Present
TS24: zona limitans intrathalamica	Present
TS24: basal forebrain	Present	Regionally restricted		Expressed in the basal plate domains of p1, p2.
TS24: midbrain	Present	Regionally restricted		Expressed in the basal plate.
TS24: spinal cord lateral wall	Present	Regionally restricted		Expressed in cells next to the floor plate.
TS24: dermis	Present	Regionally restricted		Expressed in the face, chest and back.