Probe:	Sim2 cDNA6
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	3C	Expressed in a narrow band in the caudal portion of the forebrain, immediately rostral to the midbrain forebrain boundary.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	4D	Expressed in a ventral domain of the caudal portion of the diencephalon in the basal plate.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	4F	Expressed in the caudal portion of the diencephalon in the basal plate and crosses the midline at the mammillary area.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Strong	Regionally restricted	8H	Expressed in dorsal and ventral aspects of the limb, apparently in the region of the muscle masses.

Structure	Level	Pattern	Image	Note
TS24: brain	Present		9A
TS24: limb digit	Present		9A
TS24: mandible	Present		9A
TS24: oral epithelium	Present		9A
TS24: rib	Present		9A
TS24: secondary palate	Present		9A
TS24: tongue	Present		9A
TS24: vertebra	Present		9A
TS24: renal tubule	Present	Regionally restricted	9A	Expressed in the central tubules.
TS24: trunk musculature	Present	Regionally restricted	9A	Expressed in the subsets of the trunk muscles at the limb level.

Structure	Level	Pattern	Image	Note
TS24: brain	Present		9B
TS24: limb digit	Present		9B
TS24: mandible	Present		9B
TS24: nasal meatus	Present		9B
TS24: rib	Present		9B
TS24: trachea	Present		9B
TS24: vertebra	Present		9B
TS24: trunk musculature	Present	Regionally restricted	9B	Expressed in the subsets of the trunk muscles at the limb level.
TS24: renal tubule	Present	Regionally restricted	9B	Expressed in the central tubules.

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Present	Regionally restricted		Expressed in the mediolateral dimension, being excluded from the lateral edge of the neural plate.

Structure	Level	Pattern	Image	Note
TS15: mesonephric tubule	Weak

Structure	Level	Pattern	Image	Note
TS17: diencephalon mantle layer	Present			Similar expression as in Sim1.
TS17: diencephalon ventricular layer	Present			Similar expression as in Sim1.
TS17: telencephalon	Present			Similar expression as in Sim1.
TS17: diencephalon	Present	Regionally restricted		Expression crossed the ventral midline in the mammillary area.
TS17: branchial arch mesenchyme	Strong

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Absent
TS20: hypothalamus	Present
TS20: mammillary area	Present
TS20: diencephalon mantle layer	Present
TS20: diencephalon ventricular layer	Present
TS20: muscle tissue	Present
TS20: rib pre-cartilage condensation	Present
TS20: vertebral cartilage condensation	Present

Structure	Level	Pattern	Image	Note
TS24: mammillary area	Present	Regionally restricted		Expressed in the lateral mammillary nuclei.
TS24: anterior hypothalamic nucleus	Present
TS24: cerebral cortex	Present
TS24: hyoid	Present
TS24: main olfactory bulb	Present
TS24: zona limitans intrathalamica	Present
TS24: basal forebrain	Present	Regionally restricted		Expressed in the basal plate domains of p1, p2.
TS24: midbrain	Present	Regionally restricted		Expressed in the basal plate.