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Gene Expression Data
Assay Details
Assay
Reference: J:266577 Rodrigo Albors A, et al., Lineage tracing of axial progenitors using Nkx1-2CreER(T2) mice defines their trunk and tail contributions. Development. 2018 Oct 2;145(19):dev164319
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6471022
Gene symbol: Tbx6
Gene name: T-box 6
Antibody: anti-Tbx6 (AF4744)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen S2A rostral node: embryonic day 8.5 (more )
Note: Double labeled: red - Tbx6; blue - Sox2 (assay MGI:6471020).
Structure Level Pattern Image Note
TS13: embryo ectoderm Present Regionally restricted S2A rostral node TBX6 , S2A rostral node (merge) Authors report expression in primitive streak epiblast,
Specimen S2A NSB: embryonic day 8.5 (more )
Note: Double labeled: red - Tbx6; blue - Sox2 (assay MGI:6471020).
Structure Level Pattern Image Note
TS13: embryo ectoderm Present Regionally restricted S2A NSB TBX6 , S2A NSB (merge) Authors report expression in primitive streak epiblast,
TS13: neural plate Absent S2A NSB TBX6 , S2A NSB (merge) No expression in neural plate of NSB (node-streak border) epiblast,
Specimen S2A streak and CLE: embryonic day 8.5 (more )
Note: Double labeled: red - Tbx6; blue - Sox2 (assay MGI:6471020).
Structure Level Pattern Image Note
TS13: primitive streak Present S2A streak and CLE (merge) , S2A streak and CLE TBX6
TS13: embryo ectoderm Present Scattered S2A streak and CLE TBX6 , S2A streak and CLE (merge) Expression in only a few scattered cells in the CLE (caudal lateral epiblast).
Specimen S2B (1st row): embryonic day 10.5 (more )
Note: Double labeled: red - Tbx6; blue - Sox2 (assay MGI:6471020).
Structure Level Pattern Image Note
TS17: neural tube Absent S2B 1st row TBX6 , S2B 1st row (merge)
Specimen S2B (2nd row): embryonic day 10.5 (more )
Note: Double labeled: red - Tbx6; blue - Sox2 (assay MGI:6471020).
Structure Level Pattern Image Note
TS17: neural tube Absent S2B 2nd row TBX6 , S2B 2nd row (merge)
TS17: unsegmented mesenchyme Strong S2B 2nd row (merge) , S2B 2nd row TBX6 High levels of expression in presomitic mesoderm cells.
Specimen S2B (3rd row): embryonic day 10.5 (more )
Note: Double labeled: red - Tbx6; blue - Sox2 (assay MGI:6471020).
Structure Level Pattern Image Note
TS17: embryo mesenchyme Weak Regionally restricted S2B 3rd row (merge) , S2B 3rd row TBX6 Low but detectable levels of expression in all Sox2-expressing cells in CNH (chordo-neural hinge) region, including Nkx1-2-expressing cells in neural tube (higher in ventral half) and in neuromesodermal lip.
TS17: neural tube Weak Regionally restricted S2B 3rd row (merge) , S2B 3rd row TBX6 Low but detectable levels of expression in all Sox2-expressing cells in CNH (chordo-neural hinge) region, including Nkx1-2-expressing cells in neural tube (higher in ventral half) and in neuromesodermal lip.
Specimen S2B (4th row): embryonic day 10.5 (more )
Note: Double labeled: red - Tbx6; blue - Sox2 (assay MGI:6471020).
Structure Level Pattern Image Note
TS17: tail mesenchyme Present S2B 4th row TBX6 , S2B 4th row (merge)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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