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Gene Expression Data
Assay Details
Assay
Reference: J:136726 Byrne MJ, et al., MicroRNA expression in preimplantation mouse embryos from Ped gene positive compared to Ped gene negative mice. J Assist Reprod Genet. 2008 May;25(5):205-14
Assay type: RT-PCR
MGI Accession ID: MGI:6470890
Gene symbol: Mir125b-2
Gene name: microRNA 125b-2
Probe: miR-125b forward, miR-125b reverse
Probe preparation: labelled with SYBR green
Assay notes: Real-time RT-PCR.
Results Image: 2a
 Sample Information Bands Other Sample Information
Lane AgeStructure 72 bp (a) Amount Genetic BackgroundMutant Allele(s)Sex
2-cell B6.K2 E1.0 TS2: 2-cell stage conceptus Present 0.01 µg; total RNA B6.Cg-H2b4/FlaCmwJ Not Specified
2-cell B6.K1 E1.0 TS2: 2-cell stage conceptus Present 0.01 µg; total RNA B6.Cg-H2b3/FlaCmwJ Not Specified
4-cell B6.K2 E2.0 TS3: 4-cell stage conceptus Present 0.01 µg; total RNA B6.Cg-H2b4/FlaCmwJ Not Specified
4-cell B6.K1 E2.0 TS3: 4-cell stage conceptus Present 0.01 µg; total RNA B6.Cg-H2b3/FlaCmwJ Not Specified
8-cell B6.K2 E2.5 TS3: 8-cell stage conceptus Present 0.01 µg; total RNA B6.Cg-H2b4/FlaCmwJ Not Specified
8-cell B6.K1 E2.5 TS3: 8-cell stage conceptus Present 0.01 µg; total RNA B6.Cg-H2b3/FlaCmwJ Not Specified
Morula B6.K2 E3.0 TS3: morula-stage conceptus Present 0.01 µg; total RNA B6.Cg-H2b4/FlaCmwJ Not Specified
Morula B6.K1 E3.0 TS3: morula-stage conceptus Present 0.01 µg; total RNA B6.Cg-H2b3/FlaCmwJ Not Specified
Blastocyst B6.K2 E3.5 TS5: blastocyst-stage conceptus Present 0.01 µg; total RNA B6.Cg-H2b4/FlaCmwJ Not Specified
Blastocyst B6.K1 E3.5 TS5: blastocyst-stage conceptus Present (b) 0.01 µg; total RNA B6.Cg-H2b3/FlaCmwJ Not Specified
Notes:
(a) There is no variation in amplification at any developmental stage in both B6.K1 and B6.K2 preimplantation embryos.
(b) Expression increased 10-fold in B6.K1 blastocysts compared to B6.K2 blastocysts.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory