Gata2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:176660 Abler LL, et al., A high-resolution molecular atlas of the fetal mouse lower urogenital tract. Dev Dyn. 2011 Oct;240(10):2364-77
Assay type:	RNA in situ
MGI Accession ID:	MGI:6470812
Gene symbol:	Gata2
Gene name:	GATA binding protein 2

Probe:	CVprobeGata2(0019)
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

10A ©

Specimen 10A: embryonic day 17.5 (more

)
Note: Labeling was visualized using BM purple. The specimen was also stained for Cdh1 (red) to visualize the urothelium and Acta2 (green) to visualize the muscularis mucosa and muscularis propria.

Structure	Level	Pattern	Image	Note
TS25: epithelium of pelvic urethra of male	Present	Non-Uniform	10A	Expression is detected in the dorsal aspect of the urethra but was not detected along the right-left midline of the ventral urethra at this stage.
TS25: mesenchymal layer of pelvic urethra of male	Present	Non-Uniform	10A	Expression is detected in the dorsal aspect of the urethra but was not detected along the right-left midline of the ventral urethra at this stage.
TS25: presumptive neural crest derivative of pelvic urethra of male	Present	Non-Uniform	10A	Expression is detected in the dorsal aspect of the urethra but was not detected along the right-left midline of the ventral urethra at this stage.

10A inset ©

Specimen 10A - inset: embryonic day 17.5 (more

)
Note: Labeling was visualized using BM purple. The specimen was also stained for Cdh1 (red) to visualize the urothelium and Acta2 (green) to visualize the muscularis mucosa and muscularis propria.

Structure	Level	Pattern	Image	Note
TS25: epithelium of pelvic urethra of male	Present	Non-Uniform	10A inset	Expression is detected in the dorsal aspect of the urethra but was not detected along the right-left midline of the ventral urethra at this stage.
TS25: mesenchymal layer of pelvic urethra of male	Present	Non-Uniform	10A inset	Expression is detected in the dorsal aspect of the urethra but was not detected along the right-left midline of the ventral urethra at this stage.
TS25: presumptive neural crest derivative of pelvic urethra of male	Present	Non-Uniform	10A inset	Expression is detected in the dorsal aspect of the urethra but was not detected along the right-left midline of the ventral urethra at this stage.

10B ©

Specimen 10B: embryonic day 17.5 (more

)
Note: Labeling was visualized using BM purple. The specimen was also stained for Cdh1 (red) to visualize the urothelium and Acta2 (green) to visualize the muscularis mucosa and muscularis propria.

Structure	Level	Pattern	Image	Note
TS25: epithelium of pelvic urethra of female	Present	Non-Uniform	10B	Expression is detected in the dorsal aspect of the urethra but was not detected along the right-left midline of the ventral urethra at this stage.
TS25: mesenchymal layer of pelvic urethra of female	Present	Non-Uniform	10B	Expression is detected in the dorsal aspect of the urethra but was not detected along the right-left midline of the ventral urethra at this stage.
TS25: presumptive neural crest derivative of pelvic urethra of female	Present	Non-Uniform	10B	Expression is detected in the dorsal aspect of the urethra but was not detected along the right-left midline of the ventral urethra at this stage.

10B inset ©

Specimen 10B - inset: embryonic day 17.5 (more

)
Note: Labeling was visualized using BM purple. The specimen was also stained for Cdh1 (red) to visualize the urothelium and Acta2 (green) to visualize the muscularis mucosa and muscularis propria.

Structure	Level	Pattern	Image	Note
TS25: epithelium of pelvic urethra of female	Present	Non-Uniform	10B inset	Expression is detected in the dorsal aspect of the urethra but was not detected along the right-left midline of the ventral urethra at this stage.
TS25: mesenchymal layer of pelvic urethra of female	Present	Non-Uniform	10B inset	Expression is detected in the dorsal aspect of the urethra but was not detected along the right-left midline of the ventral urethra at this stage.
TS25: presumptive neural crest derivative of pelvic urethra of female	Present	Non-Uniform	10B inset	Expression is detected in the dorsal aspect of the urethra but was not detected along the right-left midline of the ventral urethra at this stage.

Specimen Table S1 - male: embryonic day 17.5 (more

)
Note: Labeling was visualized using BM purple.

Structure	Level	Note
TS25: superficial cell layer of dorsal pelvic urethra of male	Absent	No expression in the superficial urothelium.
TS25: intermediate cell layer of dorsal pelvic urethra of male	Present	Expression in the intermediate urothelium.
TS25: basal cell layer of dorsal pelvic urethra of male	Absent	No expression in the basal urothelium.
TS25: lamina propria of dorsal pelvic urethra of male	Present	Expression in the lamina propria of mesenchyme but not in the macrophages.
TS25: muscularis mucosa of dorsal pelvic urethra of male	Absent	No expression in the muscularis mucosa of mesenchyme nor in the interstitial cells of Cajal.
TS25: submucosa of dorsal pelvic urethra of male	Present	Expression in the submucosa of mesenchyme.
TS25: muscle layer of dorsal pelvic urethra of male	Absent
TS25: superficial cell layer of ventral pelvic urethra of male	Absent	No expression in the superficial urothelium.
TS25: intermediate cell layer of ventral pelvic urethra of male	Absent	No expression in the intermediate urothelium.
TS25: basal cell layer of ventral pelvic urethra of male	Absent	No expression in the basal urothelium.
TS25: lamina propria of ventral pelvic urethra of male	Present	Expression in the lamina propria of mesenchyme but not in the macrophages.
TS25: muscularis mucosa of ventral pelvic urethra of male	Absent	No expression in the muscularis mucosa of mesenchyme nor in the interstitial cells of Cajal.
TS25: submucosa of ventral pelvic urethra of male	Present	Expression in the submucosa of mesenchyme.
TS25: muscle layer of ventral pelvic urethra of male	Absent
TS25: mesenchymal pad of pelvic urethra of male	Absent
TS25: vasculature of pelvic urethra of male	Absent	No expression in the vasculature nor in the pericytes.
TS25: presumptive neural crest derivative of pelvic urethra of male	Present
TS25: epithelium of seminal vesicle	Present
TS25: lamina propria of seminal vesicle	Present	Expression in the mesenchymal layer.
TS25: muscle of seminal vesicle	Absent
TS25: serosa of seminal vesicle	Absent	No expression in the adventitia.
TS25: vasculature of seminal vesicle	Absent
TS25: epithelium of ejaculatory duct	Absent
TS25: lamina propria of ejaculatory duct	Present	Expression in the mesenchymal layer.
TS25: muscle of ejaculatory duct	Absent
TS25: vasculature of ejaculatory duct	Absent
TS25: prostate epithelium	Absent
TS25: peri-prostatic mesenchyme	Present

Specimen Table S1 - female: embryonic day 17.5 (more

)
Note: Labeling was visualized using BM purple.

Structure	Level	Note
TS25: superficial cell layer of dorsal pelvic urethra of female	Absent	No expression in the superficial urothelium.
TS25: intermediate cell layer of dorsal pelvic urethra of female	Present	Expression in the intermediate urothelium.
TS25: basal cell layer of dorsal pelvic urethra of female	Absent	No expression in the basal urothelium.
TS25: lamina propria of dorsal pelvic urethra of female	Present	Expression in the lamina propria of mesenchyme but not in the macrophages.
TS25: muscularis mucosa of dorsal pelvic urethra of female	Absent	No expression in the muscularis mucosa of mesenchyme nor in the interstitial cells of Cajal.
TS25: submucosa of dorsal pelvic urethra of female	Present	Expression in the submucosa of mesenchyme.
TS25: muscle layer of dorsal pelvic urethra of female	Absent
TS25: superficial cell layer of ventral pelvic urethra of female	Absent	No expression in the superficial urothelium.
TS25: intermediate cell layer of ventral pelvic urethra of female	Absent	No expression in the intermediate urothelium.
TS25: basal cell layer of ventral pelvic urethra of female	Absent	No expression in the basal urothelium.
TS25: lamina propria of ventral pelvic urethra of female	Present	Expression in the lamina propria of mesenchyme but not in the macrophages.
TS25: muscularis mucosa of ventral pelvic urethra of female	Absent	No expression in the muscularis mucosa of mesenchyme nor in the interstitial cells of Cajal.
TS25: submucosa of ventral pelvic urethra of female	Present	Expression in the submucosa of mesenchyme.
TS25: muscle layer of ventral pelvic urethra of female	Absent
TS25: mesenchymal pad of pelvic urethra of female	Absent
TS25: vasculature of pelvic urethra of female	Absent	No expression in the vasculature nor in the pericytes.
TS25: presumptive neural crest derivative of pelvic urethra of female	Present
TS25: epithelium of upper vagina	Present
TS25: lamina propria of upper vagina	Present	Expression in the mesenchymal layer.
TS25: muscle of upper vagina	Absent
TS25: adventitia of upper vagina	Absent
TS25: vasculature of upper vagina	Absent
TS25: epithelium of sinovaginal bulb	Absent	No expression in the epithelial layer of lower vagina.
TS25: lamina propria of sinovaginal bulb	Present	Expression in the mesenchymal layer of lower vagina.
TS25: muscle of sinovaginal bulb	Absent	No expression in the muscle layer of lower vagina.
TS25: adventitia of sinovaginal bulb	Absent	No expression in the adventitia of lower vagina.
TS25: vasculature of sinovaginal bulb	Absent	No expression in the vasculature of lower vagina.

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