Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sor
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Masayuki Yazawa
MGI ID:	MGI:6470703
Synonyms:	Gt(ROSA)26Sortm1(CAG-cre*,-mKate2)Yzwa, Rosa26PA-Cre A20
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sor page

Germline Transmission:	Earliest citation of germline transmission: J:292311
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	Not Specified

Allele Type:    Targeted (Conditional ready, Inducible, Recombinase, Reporter) Inducer:    blue light exposure Mutation:    Insertion   Gt(ROSA)26Sor expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note chicken CAG   Mutation details: The targeting vector was designed with (from 5' to 3') a ubiquitously expressed CAG (CMV enhancer/chicken beta-actin) promoter, a frt-flanked STOP cassette (containing a neomycin resistance [neo] cassette and a polyadenylation [pA] signal). A loxP-flanked cassette including PA-Cre 3.0 followed by a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) and a pA site were placed downstream of the frt-flanked STOP cassette. PA-Cre 3.0 consists of a split Cre (59/60) with each fragment complemented with nMag or pMag (respectively) and a nuclear localization sequence (NLS) (CreN59-nMag-NLS and NLS-pMag-CreC60), separated by P2A self-cleaving peptide sequence (2A). The nMag and pMag are part of Magnets protein derived from the fragments of photoreceptor gene product Vivid (VVD) from a filamentous fungi Neurospora crassa. This floxed cassette is followed by a nuclear-localized red fluorescent protein (mKate2) followed by another pA signal. This targeting vector was inserted into the Gt(ROSA)26Sor locus. The construct was electroporated into 129Sv/Ev x C57BL/6 derived embryonic stem (ES) cells (clone A20) and correctly targeted ES cells were injected into blastocysts. (J:292311)

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	944 strains or lines available

Original:	J:292311 Morikawa K, et al., Photoactivatable Cre recombinase 3.0 for in vivo mouse applications. Nat Commun. 2020 May 1;11(1):2141
All:	1 reference(s)