Neto2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:278312 Michishita M, et al., Expression of Btcl2, a novel member of Btcl gene family, during development of the central nervous system. Brain Res Dev Brain Res. 2004 Oct 15;153(1):135-42
Assay type:	RNA in situ
MGI Accession ID:	MGI:6470547
Gene symbol:	Neto2
Gene name:	neuropilin (NRP) and tolloid (TLL)-like 2

Probe:	Btcl2 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 2D: embryonic day 15.0 (more

)

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Weak		2D
TS23: medulla oblongata	Strong	Regionally restricted	2D	Expressed in the inferior olive.
TS23: spinal cord	Present		2D

Specimen 2E: embryonic day 18.0 (more

)

Structure	Level	Image
TS26: cerebral cortex	Weak	2E
TS26: hippocampus	Weak	2E
TS26: spinal cord	Weak	2E
TS26: thalamus	Weak	2E
TS26: eye	Absent	2E

Specimen 2F: postnatal day 5 (more

)

Structure	Level	Image
TS28: cerebellum	Weak	2F
TS28: cerebral cortex	Weak	2F
TS28: hippocampus	Weak	2F
TS28: inferior colliculus	Weak	2F
TS28: main olfactory bulb	Weak	2F
TS28: mammillary body	Weak	2F
TS28: spinal cord	Weak	2F
TS28: thalamus	Weak	2F
TS28: pontine nucleus	Moderate	2F

Specimen 3I: embryonic day 15.0 (more

)

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Weak	Regionally restricted	3I	Expressed strongly in the deeper part, and weakly in the superficial part.
TS23: cerebral cortex marginal layer	Absent		3I
TS23: cortical subplate	Absent		3I

Specimen 3J: embryonic day 18.0 (more

)

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Weak	Regionally restricted	3J	Expressed strongly in the deeper part, and weakly in the superficial part.
TS26: cortical subplate	Present		3J	partial signal
TS26: cerebral cortex marginal zone	Absent		3J

Specimen 3K: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Weak	Regionally restricted	3K	Expressed in the superficial part.
TS28: cortical layer III	Weak	Regionally restricted	3K	Expressed in the superficial part.
TS28: cortical layer VI	Weak	Regionally restricted	3K	Expressed in the superficial part.
TS28: cortical layer I	Absent		3K
TS28: cortical layer V	Present		3K	partial signal

Specimen 3L: postnatal day 21 (more

)

Structure	Level	Pattern	Image	Note
TS28: neocortex	Present	Diffuse	3L
TS28: cortical layer I	Absent		3L
TS28: cortical layer II	Present		3L	partial signal
TS28: cortical layer III	Present		3L	partial signal
TS28: cortical layer IV	Present		3L	partial signal
TS28: cortical layer V	Present		3L	partial signal
TS28: cortical layer VI	Weak		3L

Specimen 4G: embryonic day 18.0 (more

)

Structure	Level	Image
TS26: hippocampus CA1	Weak	4G
TS26: dentate gyrus	Absent	4G
TS26: hippocampus CA2	Absent	4G
TS26: hippocampus CA3	Absent	4G

Specimen 4H: postnatal day 5 (more

)

Structure	Level	Image
TS28: hippocampus CA1	Weak	4H
TS28: dentate gyrus	Absent	4H
TS28: hippocampus CA2	Absent	4H
TS28: hippocampus CA3	Absent	4H

Specimen 4I: postnatal day 21 (more

)

Structure	Level	Image
TS28: hippocampus CA1	Moderate	4I
TS28: hippocampus CA3	Moderate	4I
TS28: hippocampus CA2	Weak	4I
TS28: dentate gyrus	Absent	4I

Specimen 4P: embryonic day 18.0 (more

)

Structure	Level	Pattern	Image	Note
TS26: cerebellum Purkinje cell layer	Weak		4P
TS26: cerebellum granule cell layer	Weak	Regionally restricted	4P	Expressed in the internal granular layer.
TS26: cerebellum external granule cell layer	Absent		4P

Specimen 4Q: postnatal day 5 (more

)

Structure	Level	Image
TS28: cerebellum Purkinje cell layer	Weak	4Q
TS28: cerebellum internal granule cell layer	Weak	4Q
TS28: cerebellum external granule cell layer	Absent	4Q

Specimen 4R: postnatal day 21 (more

)

Structure	Level	Image	Note
TS28: cerebellum internal granule cell layer	Moderate	4R
TS28: cerebellum Purkinje cell layer	Weak	4R	partial signal
TS28: cerebellum external granule cell layer	Absent	4R

Specimen not shown: postnatal week 8 (more

)

Structure	Level	Pattern	Image	Note
TS28: neocortex	Present	Diffuse

Specimen not shown: embryonic day 13.0 (more

)
Note: See Table 1.

Structure	Level	Pattern	Image	Note
TS21: cortical preplate	Weak
TS21: thalamus	Weak
TS21: eye	Absent
TS21: pons	Absent

Specimen not shown: embryonic day 15.0 (more

)
Note: See Table 1.

Structure	Level	Pattern	Note
TS23: hippocampus	Weak	Regionally restricted	Expressed in CA1, not CA2, CA3.
TS23: caudate-putamen	Absent
TS23: cerebellum external granule cell layer	Absent
TS23: dentate gyrus primordium	Absent
TS23: eye	Absent
TS23: pons	Weak
TS23: thalamus	Weak
TS23: cerebellum Purkinje cell cluster	Weak	Regionally restricted
TS23: main olfactory bulb mitral cell layer	Absent		No expression in the glomerular cell layer and granule cell layer.

Specimen not shown: embryonic day 18.0 (more

)
Note: See Table 1.

Structure	Level	Note
TS26: main olfactory bulb mitral cell layer	Weak
TS26: pons	Weak
TS26: thalamus	Weak
TS26: main olfactory bulb glomerular layer	Absent	No expression in the granule cell layer.
TS26: eye	Absent
TS26: caudate-putamen	Present	partial signal

Specimen not shown: postnatal day 5 (more

)
Note: See Table 1.

Structure	Level	Pattern	Image	Note
TS28: pons	Moderate
TS28: caudate-putamen	Weak
TS28: main olfactory bulb mitral cell layer	Weak
TS28: thalamus	Weak
TS28: main olfactory bulb glomerular layer	Absent
TS28: main olfactory bulb granule cell layer	Absent

Specimen not shown: postnatal day 21 (more

)
Note: See Table 1.

Structure	Level	Pattern	Image	Note
TS28: caudate-putamen	Moderate
TS28: main olfactory bulb granule cell layer	Weak
TS28: main olfactory bulb mitral cell layer	Weak
TS28: pons	Weak
TS28: thalamus	Weak
TS28: main olfactory bulb glomerular layer	Absent

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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