Neto1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:278312 Michishita M, et al., Expression of Btcl2, a novel member of Btcl gene family, during development of the central nervous system. Brain Res Dev Brain Res. 2004 Oct 15;153(1):135-42
Assay type:	RNA in situ
MGI Accession ID:	MGI:6470546
Gene symbol:	Neto1
Gene name:	neuropilin (NRP) and tolloid (TLL)-like 1

Probe:	Btcl1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 2A: embryonic day 15.0 (more

)

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Weak		2A
TS23: spinal cord	Weak		2A

Specimen 2B: embryonic day 18.0 (more

)

Structure	Level	Image
TS26: caudate-putamen	Moderate	2B
TS26: dentate gyrus	Absent	2B
TS26: eye	Absent	2B
TS26: cerebellum	Weak	2B
TS26: cortical plate	Weak	2B
TS26: hippocampus	Weak	2B
TS26: spinal cord	Present	2B

Specimen 2C: postnatal day 5 (more

)

Structure	Level	Image
TS28: cerebellum	Weak	2C
TS28: cerebral cortex	Weak	2C
TS28: main olfactory bulb	Weak	2C
TS28: thalamus	Weak	2C
TS28: pontine nucleus	Strong	2C
TS28: hippocampus	Moderate	2C
TS28: spinal cord	Present	2C

Specimen 3E: embryonic day 15.0 (more

)

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex marginal layer	Weak		3E
TS23: cortical subplate	Weak		3E
TS23: cortical plate	Present	Clusters	3E	Sparsely expressed in clumps in the superficial part.

Specimen 3F: embryonic day 18.0 (more

)

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex marginal zone	Weak		3F
TS26: cortical subplate	Weak		3F
TS26: cortical plate	Present	Clusters	3F	Sparsely expressed in clumps in the superficial part.

Specimen 3G: postnatal day 5 (more

)

Structure	Level	Pattern	Image
TS28: cortical layer I	Absent		3G
TS28: cerebral cortex	Weak	Diffuse	3G
TS28: cortical layer II	Weak	Diffuse	3G
TS28: cortical layer III	Weak	Diffuse	3G
TS28: cortical layer IV	Weak	Diffuse	3G
TS28: cortical layer V	Weak	Diffuse	3G
TS28: cortical layer VI	Weak	Diffuse	3G

Specimen 3H: postnatal day 21 (more

)

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Absent		3H
TS28: cortical layer IV	Absent		3H
TS28: neocortex	Weak	Diffuse	3H
TS28: cortical layer V	Weak		3H	Concentrated within relatively large cells.
TS28: cortical layer VI	Weak		3H	Concentrated within relatively large cells.
TS28: cortical layer II	Weak	Regionally restricted	3H	Expressed in the superficial part.
TS28: cortical layer III	Weak	Regionally restricted	3H	Expressed in the superficial part.

Specimen 4D: embryonic day 18.0 (more

)

Structure	Level	Pattern	Image	Note
TS26: hippocampus CA1	Weak	Regionally restricted	4D	Expressed in the strata pyramidalis.
TS26: hippocampus CA2	Weak	Regionally restricted	4D	Expressed in the strata pyramidalis.
TS26: hippocampus CA3	Weak	Regionally restricted	4D	Expressed in the strata pyramidalis.
TS26: dentate gyrus	Absent		4D

Specimen 4E: postnatal day 5 (more

)

Structure	Level	Image
TS28: hippocampus CA3	Moderate	4E
TS28: dentate gyrus	Weak	4E
TS28: hippocampus CA1	Weak	4E
TS28: hippocampus CA2	Weak	4E

Specimen 4F: postnatal day 21 (more

)

Structure	Level	Image
TS28: hippocampus CA3	Strong	4F
TS28: hippocampus CA1	Weak	4F
TS28: hippocampus CA2	Weak	4F
TS28: dentate gyrus	Moderate	4F

Specimen 4M: embryonic day 18.0 (more

)

Structure	Level	Pattern	Image	Note
TS26: cerebellum Purkinje cell layer	Weak		4M

Specimen 4N: postnatal day 5 (more

)

Structure	Level	Image
TS28: cerebellum Purkinje cell layer	Weak	4N
TS28: cerebellum external granule cell layer	Absent	4N
TS28: cerebellum internal granule cell layer	Absent	4N

Specimen 4O: postnatal day 21 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Absent		4O

Specimen not shown: postnatal week 8 (more

)

Structure	Level	Pattern	Note
TS28: neocortex	Present	Diffuse
TS28: cerebellum Purkinje cell layer	Absent
TS28: cortical layer I	Absent
TS28: cortical layer V	Present		Concentrated within relatively large cells.
TS28: cortical layer VI	Present		Concentrated within relatively large cells.
TS28: cortical layer II	Present	Regionally restricted	Expressed in the superficial part.
TS28: cortical layer III	Present	Regionally restricted	Expressed in the superficial part.

Specimen not shown: embryonic day 13.0 (more

)
Note: See Table 1.

Structure	Level	Pattern	Note
TS21: cortical preplate	Weak
TS21: corpus striatum	Weak	Regionally restricted	Expressed in the caudate putamen.
TS21: eye	Absent
TS21: pons	Absent
TS21: thalamus	Absent

Specimen not shown: embryonic day 15.0 (more

)
Note: See Table 1.

Structure	Level	Pattern	Note
TS23: main olfactory bulb mitral cell layer	Absent		No expression in the glomerular cell layer and granule cell layer.
TS23: hippocampus	Weak	Regionally restricted	Expressed in CA1, not CA2 and CA3.
TS23: dentate gyrus primordium	Absent
TS23: eye	Absent
TS23: caudate-putamen	Moderate
TS23: pons	Moderate
TS23: thalamus	Weak
TS23: cerebellum Purkinje cell cluster	Absent
TS23: cerebellum external granule cell layer	Absent

Specimen not shown: embryonic day 18.0 (more

)
Note: See Table 1.

Structure	Level	Note
TS26: main olfactory bulb glomerular layer	Absent	No expression in the granule cell layer.
TS26: main olfactory bulb mitral cell layer	Absent
TS26: thalamus	Weak
TS26: pons	Moderate
TS26: cerebellum granule cell layer	Absent

Specimen not shown: postnatal day 5 (more

)
Note: See Table 1.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb granule cell layer	Weak
TS28: main olfactory bulb mitral cell layer	Weak
TS28: thalamus	Weak
TS28: main olfactory bulb glomerular layer	Absent

Specimen not shown: postnatal day 21 (more

)
Note: See Table 1.

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Absent
TS28: cerebellum internal granule cell layer	Absent
TS28: main olfactory bulb glomerular layer	Absent
TS28: main olfactory bulb granule cell layer	Weak
TS28: main olfactory bulb mitral cell layer	Weak
TS28: pons	Weak
TS28: thalamus	Present
TS28: caudate-putamen	Moderate

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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