Sfrp2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:192625 Keil KP, et al., Wnt inhibitory factor 1 (Wif1) is regulated by androgens and enhances androgen-dependent prostate development. Endocrinology. 2012 Dec;153(12):6091-103
Assay type:	RNA in situ
MGI Accession ID:	MGI:6470431
Gene symbol:	Sfrp2
Gene name:	secreted frizzled-related protein 2

Probe:	Sfrp2 cDNA7
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 8D: embryonic day 18.5 (more

)
Note: Labeling was visualized using BM purple. The specimen was also stained for Cdh1 (red) to visualize the epithelium and Acta2 (green) to visualize the mesenchyme.

Structure	Level	Pattern	Image	Note
TS26: mesenchymal layer of pelvic urethra of male	Present		8D	Expression is present in urogenital sinus mesenchyme.

Specimen 8E: embryonic day 18.5; Wif1^tm1Dmth/Wif1^tm1Dmth (more

)
Note: Labeling was visualized using BM purple. The specimen was also stained for Cdh1 (red) to visualize the epithelium and Acta2 (green) to visualize the mesenchyme.

Structure	Level	Pattern	Image	Note
TS26: mesenchymal layer of pelvic urethra of male	Present		8E	Expression is present in urogenital sinus mesenchyme; the area of expression is expanded in the mutant.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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