Antibody:

anti-Gata4

Structure	Level	Pattern	Image	Note
TS6: primitive endoderm	Present		2A GATA4 upper

Structure	Level	Pattern	Image	Note
TS6: primitive endoderm	Present		2A GATA4 lower	The cells were not organized into a monolayer epithelium and some located deep inside the inner cell mass.

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		2B WT lower
TS7: parietal endoderm	Present	Single cells	2B WT lower	Only one postive cell was seen in the thin layer.

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		2B1 lower	Positive endoderm cells were misplaced and intermixed within the egg cylinder core.

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		2B2 lower	Positive endoderm cells were misplaced and intermixed within the egg cylinder core.

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		2B3 lower	Positive endoderm cells were misplaced and intermixed within the egg cylinder core.

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		2B4 lower	Positive endoderm cells were misplaced and intermixed within the egg cylinder core.

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		2B5 lower	Positive endoderm cells were misplaced and intermixed within the egg cylinder core.