Gene Expression Data
Assay Details
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Assay
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Reference:
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J:36870
Wilkinson GA, et al., Neurotrophin-3 is a survival factor in vivo for early mouse trigeminal neurons. J Neurosci. 1996 Dec 1;16(23):7661-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6468024
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Gene symbol:
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Nefm
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Gene name:
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neurofilament, medium polypeptide
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Antibody:
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anti-NF-150
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
not shown - E10.5:
embryonic day 10.5
(more  )
Note: These data are reported in Fig. 2
Specimen
not shown - E10.5:
(close  )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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These data are reported in Fig. 2
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Specimen
not shown - E10.5:
embryonic day 10.5; Ntf3tm1Lfr/ Ntf3tm1Lfr
(more )
Note: These data are reported in Fig. 2
Specimen
not shown - E10.5:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3tm1Lfr
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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These data are reported in Fig. 2
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trigeminal ganglion |
Present |
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Expression is present in neurons; the number of these neurons is the same in the mutant as in wild type.
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Specimen
not shown - E11.5:
embryonic day 11.5
(more )
Note: These data are reported in Fig. 2
Specimen
not shown - E11.5:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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These data are reported in Fig. 2
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Specimen
not shown - E11.5:
embryonic day 11.5
(more )
Note: These data are reported in Fig. 2
Specimen
not shown - E11.5:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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These data are reported in Fig. 2
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: trigeminal ganglion |
Present |
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Expression is present in neurons; the mutant has only 60% of the neurons seen in wild type.
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Specimen
1A:
embryonic day 13.5
(more )
Specimen
1A:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Specimen
1B:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3tm1Lfr
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: trigeminal ganglion |
Present |
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1B
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Expression is present in neurons; the mutant has only 30% of the neurons seen in wild type.
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Specimen
not shown - het:
embryonic day 13.5; Ntf3tm1Lfr/Ntf3 +
(more )
Specimen
not shown - het:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: trigeminal ganglion |
Present |
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Expression is present in neurons; the number of these neurons in the heterozygote is essentially the same as in wild type.
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Analysis Tools