Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:32806
Keller-Peck CR, et al., Patterns of neuronal differentiation in neural tube mutant mice: curly tail and Pax3 splotch-delayed. J Comp Neurol. 1996 May 13;368(4):516-26
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6467960
|
|
Gene symbol:
|
Nefm
|
|
Gene name:
|
neurofilament, medium polypeptide
|
|
Antibody:
|
anti-NF-M (3A10)
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
Assay notes:
|
Embryos were fixed with paraformaldehyde-lysine-periodate (PLP) fixative. They were embedded in polyester wax for sectioning.
|
|
|
Results
|
Specimen
3B:
embryonic day 14.5; Grhl3ct/ Grhl3ct
(more  )
Note: sagittal section
Specimen
3B:
(close  )
|
Genetic Background:
|
STOCK Grhl3ct/J
|
|
Mutant Allele(s):
|
Grhl3ct/Grhl3ct
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
sagittal section
|
Specimen
3C:
embryonic day 13.5; Grhl3ct/ Grhl3ct
(more )
Note: transverse section
Specimen
3C:
(close )
|
Genetic Background:
|
STOCK Grhl3ct/J
|
|
Mutant Allele(s):
|
Grhl3ct/Grhl3ct
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
transverse section
|
Specimen
3D:
embryonic day 13.5; Pax3Sp-d/ Pax3Sp-d
(more )
Note: transverse section
Specimen
3D:
(close )
|
Genetic Background:
|
C57BL/6J-Pax3Sp-d
|
|
Mutant Allele(s):
|
Pax3Sp-d/Pax3Sp-d
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
transverse section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal nerve |
Present |
|
3D
|
The ventral roots exit the affected spinal cord. However, there is no sign of dorsal root axons in these embryos.
|
|
Analysis Tools
