Gene Expression Data
Assay Details
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Assay
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Reference:
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J:32806
Keller-Peck CR, et al., Patterns of neuronal differentiation in neural tube mutant mice: curly tail and Pax3 splotch-delayed. J Comp Neurol. 1996 May 13;368(4):516-26
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6467958
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Gene symbol:
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Ncam1
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Gene name:
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neural cell adhesion molecule 1
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Antibody:
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5A5 antibody
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Embryos were fixed with paraformaldehyde-lysine-periodate (PLP) fixative. They were embedded in polyester wax for sectioning.
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Results
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Specimen
2E:
embryonic day 12.5
(more  )
Note: phenotypically normal littermate control; transverse section
Specimen
2E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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phenotypically normal littermate control; transverse section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
2E
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Expression is seen in the commissural neurons, cells that differentiate in the dorsal spinal cord and extend their axons ventrally.
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Specimen
2F:
embryonic day 12.5; Grhl3ct/ Grhl3ct
(more )
Note: transverse section
Specimen
2F:
(close )
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Genetic Background:
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STOCK Grhl3ct/J
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Mutant Allele(s):
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Grhl3ct/Grhl3ct
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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transverse section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
2F
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Expression is seen in the commissural neurons, cells that differentiate in the dorsal spinal cord and extend their axons ventrally. The ventral trajectory is remarkably faithful in affected neurons.
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Specimen
2G:
embryonic day 12.5
(more )
Note: phenotypically normal littermate control; transverse section
Specimen
2G:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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phenotypically normal littermate control; transverse section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
2G
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Expression is seen in the commissural neurons, cells that differentiate in the dorsal spinal cord and extend their axons ventrally.
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Specimen
2H:
embryonic day 12.5; Pax3Sp-d/ Pax3Sp-d
(more )
Note: transverse section
Specimen
2H:
(close )
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Genetic Background:
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C57BL/6J-Pax3Sp-d
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Mutant Allele(s):
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Pax3Sp-d/Pax3Sp-d
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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transverse section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
2H
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Expression is seen in the commissural neurons, cells that differentiate in the dorsal spinal cord and extend their axons ventrally. The ventral trajectory is remarkably faithful in affected neurons.
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Analysis Tools