Gene Expression Data
Assay Details
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Assay
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Reference:
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J:32806
Keller-Peck CR, et al., Patterns of neuronal differentiation in neural tube mutant mice: curly tail and Pax3 splotch-delayed. J Comp Neurol. 1996 May 13;368(4):516-26
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6467954
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Gene symbol:
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Rbfox3
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Gene name:
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RNA binding protein, fox-1 homolog (C. elegans) 3
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Antibody:
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Anti-NeuN (clone A60; MAB377)
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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Embryos were fixed with paraformaldehyde-lysine-periodate (PLP) fixative. They were embedded in polyester wax for sectioning.
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Results
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Specimen
1A:
embryonic day 12.5
(more  )
Note: phenotypically normal littermate control; transverse section
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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phenotypically normal littermate control; transverse section
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Specimen
1B:
embryonic day 12.5; Grhl3ct/ Grhl3ct
(more )
Note: transverse section
Specimen
1B:
(close )
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Genetic Background:
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STOCK Grhl3ct/J
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Mutant Allele(s):
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Grhl3ct/Grhl3ct
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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transverse section
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Specimen
1C:
embryonic day 14.5
(more )
Note: phenotypically normal littermate control; transverse section
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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phenotypically normal littermate control; transverse section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: alar columns |
Present |
Single cells |
1C
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The number of expressing cells has increased since E12.5, particularly in the dorsal region.
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| TS22: spinal cord |
Present |
Single cells |
1C
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The number of expressing cells has increased since E12.5, particularly in the dorsal region.
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| TS22: dorsal root ganglion |
Present |
Single cells |
1C
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Specimen
1D:
embryonic day 14.5; Grhl3ct/ Grhl3ct
(more )
Note: transverse section
Specimen
1D:
(close )
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Genetic Background:
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STOCK Grhl3ct/J
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Mutant Allele(s):
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Grhl3ct/Grhl3ct
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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transverse section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: alar columns |
Present |
Single cells |
1D
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The number of expressing cells has increased since E12.5, particularly in the dorsal region.
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| TS22: spinal cord |
Present |
Single cells |
1D
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The number of expressing cells has increased since E12.5, particularly in the dorsal region.
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| TS22: dorsal root ganglion |
Present |
Single cells |
1D
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Specimen
1E:
embryonic day 12.5
(more )
Note: phenotypically normal littermate control; transverse section
Specimen
1E:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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phenotypically normal littermate control; transverse section
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Specimen
1F:
embryonic day 12.5; Pax3Sp-d/ Pax3Sp-d
(more )
Note: transverse section
Specimen
1F:
(close )
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Genetic Background:
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C57BL/6J-Pax3Sp-d
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Mutant Allele(s):
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Pax3Sp-d/Pax3Sp-d
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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transverse section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: basal columns |
Present |
Single cells |
1F
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Uniform staining seen throughout the basal plate.
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| TS20: alar columns |
Present |
Single cells |
1F
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Although there are phenotypic issues with the alar plate, the number of expressing cells is comparable to controls.
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Specimen
1G:
embryonic day 14.5
(more )
Note: phenotypically normal littermate control; transverse section
Specimen
1G:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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phenotypically normal littermate control; transverse section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: alar columns |
Present |
Single cells |
1G
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The number of expressing cells has increased since E12.5, particularly in the dorsal region.
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| TS22: spinal cord |
Present |
Single cells |
1G
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The number of expressing cells has increased since E12.5, particularly in the dorsal region.
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| TS22: dorsal root ganglion |
Present |
Single cells |
1G
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Specimen
1H:
embryonic day 14.5; Pax3Sp-d/ Pax3Sp-d
(more )
Note: transverse section
Specimen
1H:
(close )
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Genetic Background:
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C57BL/6J-Pax3Sp-d
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Mutant Allele(s):
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Pax3Sp-d/Pax3Sp-d
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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transverse section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Single cells |
1H
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| TS22: dorsal root ganglion |
Present |
Single cells |
1H
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Although there are phenotypic issues with the dorsal root ganglion, those cells that are present within the DRG do express the antigen.
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Specimen
4A:
embryonic day 16.5
(more )
Note: phenotypically normal littermate control; transverse section
Specimen
4A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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phenotypically normal littermate control; transverse section
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Specimen
4B:
embryonic day 16.5; Grhl3ct/ Grhl3ct
(more )
Note: transverse section
Specimen
4B:
(close )
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Genetic Background:
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STOCK Grhl3ct/J
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Mutant Allele(s):
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Grhl3ct/Grhl3ct
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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transverse section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: spinal cord |
Present |
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4B
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Neurons continue to differentiate but the formation of dorsal white matter tracts is severely affected in the spina bifida mutants.
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Specimen
4C:
embryonic day 18.5
(more )
Note: phenotypically normal littermate control; transverse section
Specimen
4C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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phenotypically normal littermate control; transverse section
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Specimen
4D:
embryonic day 18.5; Grhl3ct/ Grhl3ct
(more )
Note: transverse section
Specimen
4D:
(close )
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Genetic Background:
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STOCK Grhl3ct/J
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Mutant Allele(s):
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Grhl3ct/Grhl3ct
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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transverse section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
Present |
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4D
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The spinal cord has been reduced to a fraction of its former size.
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| TS26: dorsal root ganglion |
Present |
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4D
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The dorsal root ganglion has been reduced to a fraction of its former size.
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Specimen
4E:
embryonic day 16.5
(more )
Note: phenotypically normal littermate control; transverse section
Specimen
4E:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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phenotypically normal littermate control; transverse section
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Specimen
4F:
embryonic day 16.5; Pax3Sp-d/ Pax3Sp-d
(more )
Note: transverse section
Specimen
4F:
(close )
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Genetic Background:
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C57BL/6J-Pax3Sp-d
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Mutant Allele(s):
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Pax3Sp-d/Pax3Sp-d
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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|
Note:
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transverse section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: spinal cord |
Present |
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4F
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Neurons continue to differentiate but the formation of dorsal white matter tracts is severely affected in the spina bifida mutants.
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Specimen
4G:
embryonic day 18.5
(more )
Note: phenotypically normal littermate control; transverse section
Specimen
4G:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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phenotypically normal littermate control; transverse section
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Specimen
4H:
embryonic day 18.5; Pax3Sp-d/ Pax3Sp-d
(more )
Note: transverse section
Specimen
4H:
(close )
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Genetic Background:
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C57BL/6J-Pax3Sp-d
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Mutant Allele(s):
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Pax3Sp-d/Pax3Sp-d
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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transverse section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
Present |
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4H
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The spinal cord has been reduced to rudimentary structure.
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Specimen
5A:
embryonic day 18.5; Grhl3ct/ Grhl3ct
(more )
Note: transverse section from a series of 4 sections; most rostral of the series
Specimen
5A:
(close )
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Genetic Background:
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STOCK Grhl3ct/J
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Mutant Allele(s):
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Grhl3ct/Grhl3ct
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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transverse section from a series of 4 sections; most rostral of the series
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
Present |
Single cells |
5A
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There is a paucity of labeled cells.
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Specimen
5B:
embryonic day 18.5; Grhl3ct/ Grhl3ct
(more )
Note: transverse section from a series of 4 sections; next most rostral of the series
Specimen
5B:
(close )
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Genetic Background:
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STOCK Grhl3ct/J
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Mutant Allele(s):
|
Grhl3ct/Grhl3ct
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Age:
|
embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
|
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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transverse section from a series of 4 sections; next most rostral of the series
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Specimen
5C:
embryonic day 18.5; Grhl3ct/ Grhl3ct
(more )
Note: transverse section from a series of 4 sections; next most caudal of the series
Specimen
5C:
(close )
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Genetic Background:
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STOCK Grhl3ct/J
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Mutant Allele(s):
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Grhl3ct/Grhl3ct
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Age:
|
embryonic day 18.5
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Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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transverse section from a series of 4 sections; next most caudal of the series
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Specimen
5D:
embryonic day 18.5; Grhl3ct/ Grhl3ct
(more )
Note: transverse section from a series of 4 sections; most caudal of the series
Specimen
5D:
(close )
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Genetic Background:
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STOCK Grhl3ct/J
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Mutant Allele(s):
|
Grhl3ct/Grhl3ct
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|
Age:
|
embryonic day 18.5
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|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
Other - See Notes
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Embedding:
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Paraffin
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Note:
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transverse section from a series of 4 sections; most caudal of the series
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Analysis Tools