Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Cdkn2a-CASP8*,-EGFP)3Jvd
Name:	transgene insertion 3, Jan MA van Deursen
MGI ID:	MGI:6467525
Synonyms:	INK-ATTAC-3
Transgene:	Tg(Cdkn2a-CASP8*,-EGFP)3Jvd  Location: unknown
Alliance:	Tg(Cdkn2a-CASP8*,-EGFP)3Jvd page

Strain of Origin:

FVB

Transgene Type:    Transgenic (Inserted expressed sequence, Reporter) Mutation:    Insertion   Tg(Cdkn2a-CASP8*,-EGFP)3Jvd expresses 1 gene Transgene expresses: Organism Expressed Gene Homolog in Mouse Note human CASP8 (841) Casp8  (MGI:1261423) expresses human caspase 8 fused to serial FKBPv (Phe36Val mutant FKBP) dimerization domains   Tg(Cdkn2a-CASP8*,-EGFP)3Jvd expression driven by 1 gene Transgene expression driven by: Organism Driver Gene Note mouse Cdkn2a (MGI:104738)   Mutation details: A mouse p16-INK4a (Cdkn2a) promoter drives expression of human caspase 8 fused to serial FKBPv (Phe36Val mutant FKBP) dimerization domains. A myristoylation site was included to provide membrane attachment for the caspase 8/FKBP fusion protein. An internal ribosome entry site (IRES) followed by an open reading frame (ORF) coding for enhanced green fluorescence protein (EGFP) follows the caspase 8/FKBP fusion protein encoding sequences. Expression of the fusion protein under the control of the Cdkn2a adipocyte specific promoter, together with treatment to induce dimerization of the FKBP domains, results in widespread apoptosis of in senescent, but not non-senescent cells. Two lines were generated. Line 3 contains 13 copies of the transgene inserted into a single locus. (J:178299)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available

Original:	J:178299 Baker DJ, et al., Clearance of p16Ink4a-positive senescent cells delays ageing-associated disorders. Nature. 2011 Nov 10;479(7372):232-6
All:	17 reference(s)