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Gene Expression Data
Assay Details
Assay
Reference: J:41553 Jurata LW, et al., Nuclear LIM interactor, a rhombotin and LIM homeodomain interacting protein, is expressed early in neuronal development. Proc Natl Acad Sci U S A. 1996 15;93(21):11693-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6460077
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Antibody: anti-155 kDa neurofilament
Detection system: Texas Red
Results
Specimen 18 somite stage not shown: embryonic day 9.0 (more )
Structure Level Pattern Image Note
TS14: future spinal cord Ambiguous Regionally restricted Authors report no expression in NLI (Ldb1)-expressing neurons located in peripheral most region of intermediate zone.
Specimen 4d: embryonic day 9.5 (more )
Note: Double labeled: green - NF-M (Nefm); red - NLI (Ldb1 assay MGI:6460076).
Structure Level Pattern Image Note
TS15: future spinal cord Present Regionally restricted 4d Cytoplasmic expression in neuronal cells expressing nuclear NLI (Ldb1).
Specimen E9.5 not shown: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: future hindbrain Present Regionally restricted Co-expression with NLI (Ldb1) in ventral cells of hindbrain epithelium.
Specimen 4e/g: embryonic day 10.5 (more )
Note: Double labeled: green - NF-M (Nefm); red - NLI (Ldb1 assay MGI:6460076).
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 4e , 4g Expression in motor neurons.
TS17: dorsal root ganglion Present Regionally restricted 4g , 4e Expression in sensory neuron in dorsal root ganglion.
Specimen 4h: embryonic day 10.5 (more )
Note: Double labeled: green - NF-M (Nefm); red - NLI (Ldb1 assay MGI:6460076).
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 4h No expression in medial neural tube.
Specimen E10.5 not shown: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: cranial ganglion Present
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory