Gene Expression Data
Assay Details
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Assay
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Reference:
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J:294827
Lee JM, et al., KLF10 is a modulatory factor of chondrocyte hypertrophy in developing skeleton. J Orthop Res. 2020 Sep;38(9):1987-1995
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6459913
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Gene symbol:
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Ihh
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Gene name:
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Indian hedgehog
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Antibody:
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anti-IHH (ab52919)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to fluorescein
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Results
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Specimen
2C IHH WT:
embryonic day 15.5
(more  )
Specimen
2C IHH WT:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: humerus |
Present |
Regionally restricted |
2C IHH WT (left)
,
2C IHH WT (right)
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Most expression localized to prehypertrophic zone.
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Specimen
2C IHH Mutant:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Klf10tm1Wkle/Klf10tm1Wkle
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: humerus |
Weak |
Regionally restricted |
2C IHH Mutant (left)
,
2C IHH Mutant (right)
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Substantially reduced expression in prehypertrophic zone.
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Analysis Tools
