Tg(CAG-EGFP,-mCherry,-Dnmt3a,-Dnmt3l)#Yaob
Transgene Detail
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| Symbol: |
Tg(CAG-EGFP,-mCherry,-Dnmt3a,-Dnmt3l)#Yaob |
| Name: |
transgene insertion, Yayoi Obata |
| MGI ID: |
MGI:6458812 |
| Synonyms: |
2lox |
| Transgene: |
Tg(CAG-EGFP,-mCherry,-Dnmt3a,-Dnmt3l)#Yaob Location: unknown
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| Alliance: |
Tg(CAG-EGFP,-mCherry,-Dnmt3a,-Dnmt3l)#Yaob page
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| Transgene Type: |
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Transgenic (Conditional ready, Inserted expressed sequence, Reporter) |
| Mutation: |
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Insertion
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Tg(CAG-EGFP,-mCherry,-Dnmt3a,-Dnmt3l)#Yaob expresses
2 genes
Transgene expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Dnmt3a (MGI:1261827) |
mCherry, Dnmt3a and Dnmt3l are only expressed after cre-mediated recombination removes the EGFP gene |
| mouse |
Dnmt3l (MGI:1859287) |
mCherry, Dnmt3a and Dnmt3l are only expressed after cre-mediated recombination removes the EGFP gene |
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Mutation details: The transgene insert comprises the following elements: the CAG promoter, a loxP site, the EGFP fluorescent reporter gene, poly(A) signal sequence, a loxP site, the mCherry fluorescent reporter gene, sequence coding for T2A self-cleaving peptide, the cDNA sequence for the mouse Dnmt3a2 short splice variant, sequence coding for T2A self-cleaving peptide, mouse Dnmt3l cDNA sequence, and poly(A) signal sequence. This allele expresses the EGFP gene; mCherry, Dnmt3a and Dnmt3l are only expressed after cre-mediated recombination removes the EGFP gene. Two mouse lines, ak and q, carrying an estimated 8-10 copies of the transgene, were selected for further study. This allele uses the pound # sign for when the sub-line is not defined.
(J:210999)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:210999 Hara S, et al., Forced expression of DNA methyltransferases during oocyte growth accelerates the establishment of methylation imprints but not functional genomic imprinting. Hum Mol Genet. 2014 Jul 15;23(14):3853-64 |
| All: |
2 reference(s) |
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Analysis Tools
