About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:263032 Magella B, et al., Reduced Abd-B Hox function during kidney development results in lineage infidelity. Dev Biol. 2018 Jun 15;438(2):84-93
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6457107
Gene symbol: Slc12a1
Gene name: solute carrier family 12, member 1
Antibody: NKCC2 Polyclonal antibody (18970-1-AP)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 9 E15.5 WT: embryonic day 15.5 (more )
Note: Triple labeled: red - Slc12a1; green - LTA; blue - Krt8 (assay MGI:6457096).
Structure Level Pattern Image Note
TS23: anlage of loop of Henle of cortical renal tubule Present 9_E15.5 left Labeled for only a single segment type.
Specimen 9 E15.5 mut: embryonic day 15.5; Hoxatm1.1Ssp/Hoxatm1.1Ssp, Hoxdtm1.1Ssp/Hoxdtm1.1Ssp (more )
Note: Triple labeled: red - Slc12a1; green - LTA; blue - Krt8 (assay MGI:6457096).
Structure Level Pattern Image Note
TS23: anlage of loop of Henle of cortical renal tubule Present 9_E15.5 right Mutant kidney tubules showed few examples of lineage infidelity.
Specimen 9 E16.5 WT: embryonic day 16.5 (more )
Note: Triple labeled: red - Slc12a1; green - LTA; blue - Krt8 (assay MGI:6457096).
Structure Level Pattern Image Note
TS24: anlage of loop of Henle of cortical renal tubule Present 9_E16.5 left Labeled for only a single segment type.
Specimen 9 E16.5 mut: embryonic day 16.5; Hoxatm1.1Ssp/Hoxatm1.1Ssp, Hoxdtm1.1Ssp/Hoxdtm1.1Ssp (more )
Note: Triple labeled: red - Slc12a1; green - LTA; blue - Krt8 (assay MGI:6457096).
Structure Level Pattern Image Note
TS24: anlage of loop of Henle of cortical renal tubule Present 9_E16.5 right There were some mutant tubules showing expression of markers of more than one tubule segment, but compared to E18.5 mutant kidneys there were relatively few.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/28/2024
MGI 6.13 		The Jackson Laboratory