Krt8 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:263032 Magella B, et al., Reduced Abd-B Hox function during kidney development results in lineage infidelity. Dev Biol. 2018 Jun 15;438(2):84-93
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6457088
Gene symbol:	Krt8
Gene name:	keratin 8

Antibody:	TROMA 1
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

5_left ©

Specimen 5 WT: embryonic day 18.5 (more

)
Note: Triple labeled: green - Krt8; red - DBA; purple - Rpe65 (assay MGI:6457089).

Structure	Level	Image
TS26: collecting duct	Present	5_left
TS26: pelvic urothelial lining	Present	5_left
TS26: urothelium of ureter	Present	5_left

5_right ©

Specimen 5 mut: embryonic day 18.5; Hoxa^tm1.1Ssp/Hoxa^tm1.1Ssp, Hoxd^tm1.1Ssp/Hoxd^tm1.1Ssp (more

)
Note: Triple labeled: green - Krt8; red - DBA; purple - Rpe65 (assay MGI:6457089).

Structure	Level	Image	Note
TS26: pelvic urothelial lining	Present	5_right	The open pelvic space present in wild type kidneys was absent.
TS26: collecting duct	Present	5_right
TS26: urothelium of ureter	Present	5_right

6_Krt8 left ©

6_merge left ©

Specimen 6 WT: embryonic day 18.5 (more

)
Note: Multi-labeled: green - Krt8; blue - Hnf4a (assay MGI:6457109); purple - Slc12a1 (assay MGI:6457105).

Structure	Level	Pattern	Image	Note
TS26: collecting duct	Present		6_merge left , 6_Krt8 left	The cells of each tubule labeled with a single segment marker.

6_Krt8 right ©

Specimen 6 mut: embryonic day 18.5; Hoxa^tm1.1Ssp/Hoxa^tm1.1Ssp, Hoxd^tm1.1Ssp/Hoxd^tm1.1Ssp (more

)
Note: Multi-labeled: green - Krt8; blue - Hnf4a (assay MGI:6457109); purple - Slc12a1 (assay MGI:6457105).

Structure	Level	Pattern	Image	Note
TS26: renal proximal tubule	Present		6_merge right , 6_Krt8 right	Also labeled with Slc12a1 or Hnf4a.

S3_left ©

Specimen S3 WT: embryonic day 18.5 (more

)
Note: Triple labeled: green - Krt8; red - DBA; purple - Hnf4a (assay MGI:6457110).

Structure	Level	Pattern	Image	Note
TS26: collecting duct	Present		S3_left	Not colocalized with Hnf4a.

S3_right ©

Specimen S3 mut: embryonic day 18.5; Hoxa^tm1.1Ssp/Hoxa^tm1.1Ssp, Hoxd^tm1.1Ssp/Hoxd^tm1.1Ssp (more

)
Note: Triple labeled: green - Krt8; red - DBA; purple - Hnf4a (assay MGI:6457110).

Structure	Level	Pattern	Image	Note
TS26: collecting duct	Present		S3_right	Coexpressed with Hnf4a.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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