Antibody:	anti-tPA (H-90; sc-15346)
Detection system:	Secondary antibody coupled to Alexa Fluor 568

Structure	Level	Pattern	Image	Note
TS15: surface ectoderm	Present	Widespread	2A	Expression is detected throughout the head ectoderm.
TS15: lens placode	Present		2A
TS15: optic vesicle	Present		2A
TS15: head mesenchyme	Present		2A

Structure	Level	Pattern	Image	Note
TS17: lens pit	Present	Punctate	2B	Expression is highly localized near the apical surface.
TS17: optic cup inner layer	Present	Punctate	2B	Expression is localized near the apical surface of the prospective neural retina.
TS17: optic cup outer layer	Present	Punctate	2B	Expression is localized near the apical surface of the prospective pigmented retinal epithelium.

Structure	Level	Pattern	Image	Note
TS19: neural retina	Present	Punctate	2D	Expression is localized near the apical surface.
TS19: pigmented retinal epithelium	Present	Punctate	2D	Expression is localized near the apical surface.

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Present	Punctate	2E	Expression is localized near the apical surface.

Structure	Level	Pattern	Image	Note
TS27: lens epithelium	Present		3A	Expression is seen at the apical borders.
TS27: lens fiber	Present		3A	Expression is seen at the apical borders.

Structure	Level	Pattern	Image	Note
TS28: lens epithelium	Present	Regionally restricted	3D	Expression is seen in the central lens epithelium.

Structure	Level	Pattern	Image	Note
TS28: lens equatorial epithelium	Absent		3G	There is no detectable signal in the equatorial epithelium.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Punctate	4C , 4B	Expression is maintained in a vesicular pattern at the apical-apical interface of the retinal pigmented epithelium and the neuroepithelium.
TS24: pigmented retinal epithelium	Present	Punctate	4C , 4B	Expression is maintained in a vesicular pattern at the apical-apical interface of the retinal pigmented epithelium and the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS28: neural retina	Present	Punctate	4F , 4G	Expression is maintained in a vesicular pattern at the apical-apical interface of the retinal pigmented epithelium and the neuroepithelium.
TS28: pigmented retinal epithelium	Present	Punctate	4F , 4G	Expression is maintained in a vesicular pattern at the apical-apical interface of the retinal pigmented epithelium and the neuroepithelium.
TS28: retina outer plexiform layer	Present		4G , 4F

Structure	Level	Pattern	Image	Note
TS28: neural retina	Present	Punctate	4J , 4K	Expression is maintained in a vesicular pattern at the apical-apical interface of the retinal pigmented epithelium and the neuroepithelium.
TS28: pigmented retinal epithelium	Present	Punctate	4J , 4K	Expression is maintained in a vesicular pattern at the apical-apical interface of the retinal pigmented epithelium and the neuroepithelium.
TS28: retina outer nuclear layer	Present		4J , 4K

Structure	Level	Pattern	Image	Note
TS28: neural retina	Trace
TS28: pigmented retinal epithelium	Trace
TS28: retina outer nuclear layer	Trace