Cacna1s RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:181134 Tang ZZ, et al., Muscle weakness in myotonic dystrophy associated with misregulated splicing and altered gating of CaV1.1 calcium channel. Hum Mol Genet. 2012 Mar 15;21(6):1312-24
Assay type:	RT-PCR
MGI Accession ID:	MGI:6456560
Gene symbol:	Cacna1s
Gene name:	calcium channel, voltage-dependent, L type, alpha 1S subunit

Probe:	CaV1.2-E33-Fwd, CaV1.2-E33-Rev
Assay notes:	Primers were 5' end labeled with 56-FAM.

Results

Image: 4B CaV1.2-E33

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Amount	Genetic Background	Mutant Allele(s)	Sex
WT	P m 6	TS28: heart ventricle	Strong (b)	Not Specified; poly-A+ RNA	involves: 129S1/Sv		Male
WT	P m 6	TS28: heart ventricle	Strong (c)	Not Specified; poly-A+ RNA	involves: 129S1/Sv		Male
Mbnl1+/deltaE3	P m 6	TS28: heart ventricle	Strong (b)	Not Specified; poly-A+ RNA	involves: 129S1/Sv	Mbnl1^tm1Sws/Mbnl1⁺	Male
Mbnl1+/deltaE3	P m 6	TS28: heart ventricle	Strong (b)	Not Specified; poly-A+ RNA	involves: 129S1/Sv	Mbnl1^tm1Sws/Mbnl1⁺	Male
Mbnl1deltaE3/deltaE3	P m 6	TS28: heart ventricle	Strong (b)	Not Specified; poly-A+ RNA	involves: 129S1/Sv	Mbnl1^tm1Sws/Mbnl1^tm1Sws	Male
Mbnl1deltaE3/deltaE3	P m 6	TS28: heart ventricle	Strong (c)	Not Specified; poly-A+ RNA	involves: 129S1/Sv	Mbnl1^tm1Sws/Mbnl1^tm1Sws	Male
Mbnl1deltaE3/deltaE3	P m 6	TS28: heart ventricle	Strong (c)	Not Specified; poly-A+ RNA	involves: 129S1/Sv	Mbnl1^tm1Sws/Mbnl1^tm1Sws	Male

Notes:
(a) There are two bands, a strong band that included exon 33 and a faint band that skipped exon 33.
(b) 97% of the muscle included exon 33.
(c) 96% of the muscle included exon 33.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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