Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(CAG-mRuby2*,-mClover*)1Srgt
Name:	transgene insertion 1, Sergi Regot
MGI ID:	MGI:6455404
Synonyms:	ERK-KTRLSL
Transgene:	Tg(CAG-mRuby2*,-mClover*)1Srgt  Location: unknown  Genetic Position: Chr13, Syntenic
Alliance:	Tg(CAG-mRuby2*,-mClover*)1Srgt page

Strain of Origin:

(C57BL/6 x SJL)F1

Transgene Type:    Transgenic (Reporter) Mutation:    Insertion   Mutation details: CRISPR/cas9 endonuclease-mediated genome editing was used to introduce a CMV enhancer/chicken beta-actin (CAG) promoter followed by a loxP-flanked STOP cassette, an H2B/mRuby2 fusion protein, a P2A self-cleaving peptide sequence, and an ERK-KTR/mClover fusion protein into the Gt(ROSA)26Sor locus. ERK-KTR consists of a docking site (a fragment of human ELK1, an ERK substrate), nuclear localization (NLS) and export (NES) signals, and mClover green/yellow fluorescent protein. Through nanopore sequencing and PCR verification, it was determined that the vector, along with the Rosa26 homology arms, randomly inserted into a noncoding region of chromosome 13. (J:297084)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:297084 Pokrass MJ, et al., Cell-Cycle-Dependent ERK Signaling Dynamics Direct Fate Specification in the Mammalian Preimplantation Embryo. Dev Cell. 2020 Nov 9;55(3):328-340.e5
All:	1 reference(s)