Gene Expression Data
Assay Details
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Assay
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Reference:
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J:162174
Zizic Mitrecic M, et al., The mouse gene Noto is expressed in the tail bud and essential for its morphogenesis. Cells Tissues Organs. 2010 Jul;192(2):85-92
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6452960
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Gene symbol:
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Noto
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Gene name:
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notochord homeobox
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Reporter:
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GFP
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Antibody:
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anti-GFP (#A-6455)
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Detection system:
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Alexa Fluor 594
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Assay notes:
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Specimens were fixed in 1% paraformaldehyde:1% glutaraldehyde, and embedded in Durcopan (Fluka).
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Results
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Specimen
3:
embryonic day 11.5; Nototm1Gos/Noto +
(more  )
Specimen
3:
(close  )
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Genetic Background:
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involves: 129S1/SvImJ
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Mutant Allele(s):
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Nototm1Gos/Noto+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: tail |
Present |
Regionally restricted |
3b/c
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Expression in cells at tip of tail.
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Specimen
4:
embryonic day 11.5; Nototm1Gos/Noto +
(more )
Specimen
4:
(close )
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Genetic Background:
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involves: 129S1/SvImJ
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Mutant Allele(s):
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Nototm1Gos/Noto+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: tail mesenchyme |
Present |
Regionally restricted |
4b/c
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In more cranial sections, expressing cells in dorsal portion of tail cord.
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Specimen
5:
embryonic day 11.5; Nototm1Gos/Noto +
(more )
Specimen
5:
(close )
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Genetic Background:
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involves: 129S1/SvImJ
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Mutant Allele(s):
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Nototm1Gos/Noto+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: notochord |
Present |
Regionally restricted |
5b/c
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In sections further cranial, expressing cells in notochord.
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Specimen
10:
(close )
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Genetic Background:
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involves: 129S1/SvImJ
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Mutant Allele(s):
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Nototm1Gos/Nototm1Gos
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: tail mesenchyme |
Present |
Regionally restricted |
10b/c
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Expression in cells distributed within tail bud mesenchyme.
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Specimen
11:
(close )
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Genetic Background:
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involves: 129S1/SvImJ
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Mutant Allele(s):
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Nototm1Gos/Nototm1Gos
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: tail mesenchyme |
Present |
Regionally restricted |
11
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Expression in small portion of cells with additional lumen resembling the notochord.
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| TS19: tail somite |
Present |
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11
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Expression within differentiating somites.
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Analysis Tools