Probe:	Scube2 probe1
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	2A	Expression in wall of third ventricle.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	2B	Expression in wall of third ventricle.
TS19: neural tube	Present		2B

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present		2C

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	2D	Expression in wall of fourth ventricle.
TS20: chondrocranium	Present	Regionally restricted	2D	Expression in cartilaginous primordium of basioccipital bone (clivus).
TS20: C1 vertebral cartilage condensation	Present		2D
TS20: C2 vertebral cartilage condensation	Present		2D

Structure	Level	Pattern	Image	Note
TS20: C1 vertebral cartilage condensation	Present		2E
TS20: C2 vertebral cartilage condensation	Present		2E
TS20: C3 vertebral cartilage condensation	Present		2E
TS20: C4 vertebral cartilage condensation	Present		2E

Structure	Level	Pattern	Image	Note
TS20: C1 vertebral cartilage condensation	Present		2F
TS20: C2 vertebral cartilage condensation	Present		2F

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	2G	Expression in wall of third ventricle.

Structure	Level	Pattern	Image	Note
TS20: C2 vertebral cartilage condensation	Present	Regionally restricted	2H
TS20: C3 vertebral cartilage condensation	Present	Regionally restricted	2H
TS20: C4 vertebral cartilage condensation	Present	Regionally restricted	2H
TS20: spinal cord	Present	Regionally restricted	2H

Structure	Level	Pattern	Image	Note
TS20: C2 vertebral cartilage condensation	Present		2I
TS20: C3 vertebral cartilage condensation	Present		2I
TS20: C4 vertebral cartilage condensation	Present		2I
TS20: spinal cord	Present		2I

Structure	Level	Pattern	Image	Note
TS20: epithalamus	Present		3A	Expression in roof of third ventricle in epithalamic region.

Structure	Level	Pattern	Image	Note
TS21: epithalamus	Present		3B	Expression in roof of third ventricle in epithalamic region.

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Present		3C

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Present		3D

Structure	Level	Pattern	Image	Note
TS23: epithalamus	Strong	Regionally restricted	3E	Strong and restricted expression in lateral region of epithalamus.

Structure	Level	Pattern	Image	Note
TS23: nasal septum	Strong		3F

Structure	Level	Pattern	Image	Note
TS23: tongue mesenchyme	Strong		3G

Structure	Level	Pattern	Image	Note
TS24: nasal septum	Strong		3H

Structure	Level	Pattern	Image	Note
TS24: nasal septum	Strong		3I

Structure	Level	Pattern	Image	Note
TS24: genioglossus	Strong		3J
TS24: tongue mesenchyme	Strong		3J

Structure	Level	Pattern	Image	Note
TS25: tongue mesenchyme	Strong		3K

Structure	Level	Pattern	Image	Note
TS25: genioglossus	Strong		3L
TS25: tongue mesenchyme	Strong		3L

Structure	Level	Pattern	Image	Note
TS22: dorsal aorta	Strong		4A	Strong expression localized in major blood vessels of the heart.
TS22: pulmonary artery	Strong		4A	Strong expression localized in major blood vessels of the heart.
TS22: pulmonary trunk	Strong		4A	Strong expression localized in major blood vessels of the heart.
TS22: vena cava	Strong		4A	Strong expression localized in major blood vessels of the heart.
TS22: heart ventricle	Present		4A
TS22: axial skeleton thoracic region	Present	Regionally restricted	4A	Expression in costal cartilages

Structure	Level	Pattern	Image	Note
TS23: pulmonary artery	Strong		4B	Strong expression in major blood vessels of the heart
TS23: pulmonary trunk	Strong		4B	Strong expression in major blood vessels of the heart
TS23: thoracic aorta	Strong		4B	Strong expression in major blood vessels of the heart
TS23: vena cava	Strong		4B	Strong expression in major blood vessels of the heart
TS23: heart ventricle	Present		4B
TS23: costal cartilage	Present		4B

Structure	Level	Pattern	Image	Note
TS24: thoracic aorta	Strong		4C
TS24: vena cava	Strong		4C
TS24: costal cartilage	Present	Regionally restricted	4C	Expression in costal cartilages, restricted to perichondrial layer.

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Strong		5A

Structure	Level	Pattern	Image	Note
TS22: lumbar vertebral cartilage condensation	Strong	Regionally restricted	5B	Strong expression in lumbar vertebrae, restricted to perichondrial layer.

Structure	Level	Pattern	Image	Note
TS23: radius	Present	Regionally restricted	5C	Expression in forelimb surrounding radius and ulna, restricted to perichondrial layer.
TS23: ulna	Present	Regionally restricted	5C	Expression in forelimb surrounding radius and ulna, restricted to perichondrial layer.

Structure	Level	Pattern	Image	Note
TS24: fibula	Present	Regionally restricted	5D	Expression in hindlimb in fibula and tibia, restricted to perichondrial layer.
TS24: tibia	Present	Regionally restricted	5D	Expression in hindlimb in fibula and tibia, restricted to perichondrial layer.