Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:33880
Hakem R, et al., The tumor suppressor gene Brca1 is required for embryonic cellular proliferation in the mouse. Cell. 1996 Jun 28;85(7):1009-23
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6452468
|
|
Gene symbol:
|
Ccne1
|
|
Gene name:
|
cyclin E1
|
|
Antibody:
|
anti-cyclin E
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin
|
|
|
Results
|
Specimen
6D:
embryonic day 6.5
(more  )
Note: wild-type littermate
Specimen
6D:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 6.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
wild-type littermate
|
Specimen
6E:
(close )
|
Genetic Background:
|
either: (involves: 129P2/OlaHsd * C3H/HeJ) or (involves: 129P2/OlaHsd * C57BL/6J) or (involves: 129P2/OlaHsd * CD-1)
|
|
Mutant Allele(s):
|
Brca1tm1Mak/Brca1tm1Mak
|
|
Age:
|
embryonic day 6.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo |
Present |
|
6E
|
Less severe mutant phenotype. Lower proportion (50-60%) of expressing cells in embryo.
|
| TS9: trophoblast giant cell |
Strong |
|
6E
|
Strong expression in giant cells, comparable to wild type.
|
Specimen
6F:
(close )
|
Genetic Background:
|
either: (involves: 129P2/OlaHsd * C3H/HeJ) or (involves: 129P2/OlaHsd * C57BL/6J) or (involves: 129P2/OlaHsd * CD-1)
|
|
Mutant Allele(s):
|
Brca1tm1Mak/Brca1tm1Mak
|
|
Age:
|
embryonic day 6.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo |
Weak |
|
6F
|
More severe mutant phenotype. Weakly expressing cells seen in embryo.
|
| TS9: trophoblast giant cell |
Strong |
|
6F
|
Strong expression in giant cells, comparable to wild type.
|
|
Analysis Tools