Six1 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:158421 Coletta RD, et al., Characterization of the Six1 homeobox gene in normal mammary gland morphogenesis. BMC Dev Biol. 2010;10:4
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:6452402
Gene symbol:	Six1
Gene name:	sine oculis-related homeobox 1

Reporter:	GFP
Antibody:	anti-GFP (Chemicon)
Detection system:	Secondary antibody coupled to fluorescein

Results

1B E17.5 top ©

Specimen 1B top: embryonic day 17.5; Six1^tm1Kwk/Six1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS25: mammary gland primordium epithelium	Strong	Regionally restricted	1B E17.5 top	Expression is in the invaginating epithelial cells.
TS25: mammary gland primordium	Strong	Regionally restricted	1B E17.5 top	Expression is in the muscle.

Specimen 1B bottom: embryonic day 17.5; Six1^tm1Kwk/Six1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS25: mammary gland primordium epithelium	Strong	Regionally restricted	1B E17.5 bottom	Expression is in the invaginating epithelial cells.
TS25: mammary gland primordium	Strong	Regionally restricted	1B E17.5 bottom	Expression is in the muscle.

Specimen 1B 5 Wk: postnatal week 5; Six1^tm1Kwk/Six1⁺ (more

)
Note: Virgin mouse.

Structure	Level	Pattern	Image	Note
TS28: mammary gland epithelium	Present		1B 5 Week Virgin

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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