About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:166819 Zagami CJ, et al., Molecular characterization of the mouse superior lateral parabrachial nucleus through expression of the transcription factor Runx1. PLoS One. 2010;5(11):e13944
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6451626
Gene symbol: Runx1
Gene name: runt related transcription factor 1
Reporter: lacZ
Antibody: anti-B-galactosidase (55976)
Results
Specimen S2: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - TIP39 (Pth2; assay MGI:6451648).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted S2 Expression in rostral hindbrain dorsocaudal to the medial paralemniscal nucleus (MPL). No overlap with Tip39 (Pth2) expression.
Specimen 3A2/A3: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Runx1 (assay MGI:6451636).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 3A3 , 3A2 Co-expression of reporter with Runx1 protein in dorsolateral rostral hindbrain.
Specimen 3B2/B3: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - NeuN (Rbfox3; assay MGI:6451637).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 3B2 , 3B3 Co-expression of reporter with Runx1 protein in dorsolateral rostral hindbrain.
Specimen S3B/D: postnatal day 20; Runx1tm2Spe/Runx1+ (more )
Note: Triple labeled: green - Runx1 reporter; red - Vglut2 (Slc17a6; assay MGI:6451649); blue - Map2 (assay MGI:6451650).
Structure Level Pattern Image Note
TS28: hindbrain Present Regionally restricted S3B , S3D Expression in dorsolateral rostral hindbrain within region expressing neuronal marker Map2.
Specimen 3C2/C3: embryonic day 12.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Mki67 (assay MGI:6451638).
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 3C3 , 3C2 No overlap of expression with Ki67 (Mki67) in dorsolateral rostral hindbrain.
Specimen 4A2: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Pvalb (assay MGI:6451640).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 4A2 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
TS26: dorsal nucleus of lateral lemniscus Absent 4A2 Expression does not overlap with Pvalb in DLL or ventral nucleus of lateral lemniscus (VLL).
TS26: ventral nucleus of lateral lemniscus Absent 4A2 Expression does not overlap with Pvalb in DLL or ventral nucleus of lateral lemniscus (VLL).
Specimen 4A3: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Pvalb (assay MGI:6451640).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 4A3 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
Specimen 4B1: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Pvalb (assay MGI:6451640).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 4B1 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
TS26: dorsal nucleus of lateral lemniscus Absent 4B1 Expression does not overlap with Pvalb in DLL or intermediate nucleus of lateral lemniscus (VLL).
TS26: intermediate nucleus of lateral lemniscus Absent 4B1 Expression does not overlap with Pvalb in DLL or intermediate nucleus of lateral lemniscus (VLL).
Specimen 4B2: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Pvalb (assay MGI:6451640).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 4B2 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
TS26: dorsal nucleus of lateral lemniscus Absent 4B2 Expression does not overlap with Pvalb in DLL.
Specimen 4B3: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Pvalb (assay MGI:6451640).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 4B3 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
Specimen 4C1: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Pax7 (assay MGI:6451642).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 4C1 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
TS26: dorsal nucleus of lateral lemniscus Absent 4C1 Expression does not overlap with Pvalb in DLL.
Specimen 4C2: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Pax7 (assay MGI:6451642).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 4C2 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
TS26: dorsal nucleus of lateral lemniscus Absent 4C2 Expression does not overlap with Pvalb in DLL.
Specimen 5A1: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Lmx1b (assay MGI:6451643).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 5A1 Expression in cells dorsal to Lmx1b-expressing cells. No overlap in expression with Lmx1b in dorsolateral rostral hindbrain.
Specimen 5A2: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Lmx1b (assay MGI:6451643).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 5A2 Expression in cells dorsal to Lmx1b-expressing cells. No overlap in expression with Lmx1b in dorsolateral rostral hindbrain.
Specimen 5B2/B3: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Cck (assay MGI:6451644).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 5B3 , 5B2 Expression overlapping with Cck in same region within dorsolateral rostral hindbrain.
Specimen 5B3 inset: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Cck (assay MGI:6451644).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 5B3 inset Expression overlapping with Cck in same region within dorsolateral rostral hindbrain.
Specimen 7A1: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Th (assay MGI:6451645).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 7A1 Expression in cells mostly dorsal to Th-expressing cells. No overlap Th in dorsolateral rostral hindbrain.
Specimen 7A2: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Th (assay MGI:6451645).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 7A2 Expression in cells mostly dorsal to Th-expressing cells. No overlap Th in dorsolateral rostral hindbrain.
Specimen 7B: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - serotonin.
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 7B Expression in partially situated within region expressing serotonin. No overlap serotonin in dorsolateral rostral hindbrain.
Specimen 8A1: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Tle1 (assay MGI:6451646).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 8A1 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
Specimen 8A3/A4: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Tle1 (assay MGI:6451646).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 8A4 , 8A3 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
Specimen 8B1: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Tle4 (assay MGI:6451647).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 8B1 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
Specimen 8B3/B4: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Tle4 (assay MGI:6451647).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 8B3 , 8B4 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory