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Gene Expression Data
Assay Details
Assay
Reference: J:166819 Zagami CJ, et al., Molecular characterization of the mouse superior lateral parabrachial nucleus through expression of the transcription factor Runx1. PLoS One. 2010;5(11):e13944
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6451626
Gene symbol: Runx1
Gene name: runt related transcription factor 1
Reporter: lacZ
Antibody: anti-B-galactosidase (55976)
Results
Specimen S2: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - TIP39 (Pth2; assay MGI:6451648).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted S2 Expression in rostral hindbrain dorsocaudal to the medial paralemniscal nucleus (MPL). No overlap with Tip39 (Pth2) expression.
Specimen 3A2/A3: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Runx1 (assay MGI:6451636).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 3A3 , 3A2 Co-expression of reporter with Runx1 protein in dorsolateral rostral hindbrain.
Specimen 3B2/B3: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - NeuN (Rbfox3; assay MGI:6451637).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 3B2 , 3B3 Co-expression of reporter with Runx1 protein in dorsolateral rostral hindbrain.
Specimen S3B/D: postnatal day 20; Runx1tm2Spe/Runx1+ (more )
Note: Triple labeled: green - Runx1 reporter; red - Vglut2 (Slc17a6; assay MGI:6451649); blue - Map2 (assay MGI:6451650).
Structure Level Pattern Image Note
TS28: hindbrain Present Regionally restricted S3B , S3D Expression in dorsolateral rostral hindbrain within region expressing neuronal marker Map2.
Specimen 3C2/C3: embryonic day 12.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Mki67 (assay MGI:6451638).
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 3C3 , 3C2 No overlap of expression with Ki67 (Mki67) in dorsolateral rostral hindbrain.
Specimen 4A2: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Pvalb (assay MGI:6451640).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 4A2 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
TS26: dorsal nucleus of lateral lemniscus Absent 4A2 Expression does not overlap with Pvalb in DLL or ventral nucleus of lateral lemniscus (VLL).
TS26: ventral nucleus of lateral lemniscus Absent 4A2 Expression does not overlap with Pvalb in DLL or ventral nucleus of lateral lemniscus (VLL).
Specimen 4A3: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Pvalb (assay MGI:6451640).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 4A3 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
Specimen 4B1: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Pvalb (assay MGI:6451640).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 4B1 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
TS26: dorsal nucleus of lateral lemniscus Absent 4B1 Expression does not overlap with Pvalb in DLL or intermediate nucleus of lateral lemniscus (VLL).
TS26: intermediate nucleus of lateral lemniscus Absent 4B1 Expression does not overlap with Pvalb in DLL or intermediate nucleus of lateral lemniscus (VLL).
Specimen 4B2: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Pvalb (assay MGI:6451640).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 4B2 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
TS26: dorsal nucleus of lateral lemniscus Absent 4B2 Expression does not overlap with Pvalb in DLL.
Specimen 4B3: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Pvalb (assay MGI:6451640).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 4B3 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
Specimen 4C1: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Pax7 (assay MGI:6451642).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 4C1 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
TS26: dorsal nucleus of lateral lemniscus Absent 4C1 Expression does not overlap with Pvalb in DLL.
Specimen 4C2: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Pax7 (assay MGI:6451642).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 4C2 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
TS26: dorsal nucleus of lateral lemniscus Absent 4C2 Expression does not overlap with Pvalb in DLL.
Specimen 5A1: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Lmx1b (assay MGI:6451643).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 5A1 Expression in cells dorsal to Lmx1b-expressing cells. No overlap in expression with Lmx1b in dorsolateral rostral hindbrain.
Specimen 5A2: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Lmx1b (assay MGI:6451643).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 5A2 Expression in cells dorsal to Lmx1b-expressing cells. No overlap in expression with Lmx1b in dorsolateral rostral hindbrain.
Specimen 5B2/B3: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Cck (assay MGI:6451644).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 5B3 , 5B2 Expression overlapping with Cck in same region within dorsolateral rostral hindbrain.
Specimen 5B3 inset: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Cck (assay MGI:6451644).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 5B3 inset Expression overlapping with Cck in same region within dorsolateral rostral hindbrain.
Specimen 7A1: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Th (assay MGI:6451645).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 7A1 Expression in cells mostly dorsal to Th-expressing cells. No overlap Th in dorsolateral rostral hindbrain.
Specimen 7A2: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Th (assay MGI:6451645).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 7A2 Expression in cells mostly dorsal to Th-expressing cells. No overlap Th in dorsolateral rostral hindbrain.
Specimen 7B: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - serotonin.
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 7B Expression in partially situated within region expressing serotonin. No overlap serotonin in dorsolateral rostral hindbrain.
Specimen 8A1: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Tle1 (assay MGI:6451646).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 8A1 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
Specimen 8A3/A4: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Tle1 (assay MGI:6451646).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 8A4 , 8A3 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
Specimen 8B1: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Tle4 (assay MGI:6451647).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 8B1 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
Specimen 8B3/B4: embryonic day 18.5; Runx1tm2Spe/Runx1+ (more )
Note: Double labeled: green - Runx1 reporter; red - Tle4 (assay MGI:6451647).
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 8B3 , 8B4 Expression in cells located ventromedial to dorsal nucleus of lateral lemniscus (DLL).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory