Summary | Mutation origin | Mutation description | Expression | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Oprm1tm1.1(cre/GFP)Rpa
Name:	opioid receptor, mu 1; targeted mutation 1.1, Richard D Palmiter
MGI ID:	MGI:6451472
Synonyms:	Oprm1Cre, Oprm1Cre:GFP
Gene:	Oprm1  Location: Chr10:6708593-6988209 bp, + strand  Genetic Position: Chr10, 1.85 cM, cytoband A2
Alliance:	Oprm1tm1.1(cre/GFP)Rpa page

Germline Transmission:	Earliest citation of germline transmission: J:325015
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1
Project Collection:	Sanger miRNA knockouts

Allele Type:    Targeted (Null/knockout, Recombinase, Reporter) Mutations:    Insertion, Intragenic deletion   Oprm1tm1.1(cre/GFP)Rpa expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Oprm1 (MGI:97441)   Mutation details: A cassette encoding Cre:GFP (a Myc-tag and nuclear localization signals at the N-terminus of Cre recombinase, which is fused to green fluorescent protein followed by a SV40 polyadenylation sequence) was inserted just 5' of the initiation codon in the first coding exon of the Oprm1 gene replacing the exon 1 via homologous recombination. The neomycin cassette was excised of through crosses with animals broadly expressing Flp recombinase. (J:314358, J:325015)

Activity:	Tissue activity of this recombinase allele
Driver:	Oprm1 (mouse) Summary of all recombinase alleles driven by Oprm1. Comparative matrix view of recombinase activities Alliance mouse Oprm1 gene page

In Mice Carrying this Mutation:

3 assay results

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Oprm1 Mutation:	53 strains or lines available

Original:	J:325015 Liu S, et al., Divergent brainstem opioidergic pathways that coordinate breathing with pain and emotions. Neuron. 2022 Mar 2;110(5):857-873.e9
All:	5 reference(s)