Gene Expression Data
Assay Details
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Assay
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Reference:
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J:289436
Wang W, et al., Spatial and temporal deletion reveals a latent effect of Megf8 on the left-right patterning and heart development. Differentiation. 2020 May - Jun;113:19-25
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6451076
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Gene symbol:
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Megf8
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Gene name:
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multiple EGF-like-domains 8
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Tissue fixed in 2% formaldehyde containing 0.2% glutaraldehyde.
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Results
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Specimen
1B:
embryonic day 6.5; Megf8tm1Nju/Megf8 +
(more  )
Specimen
1B:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Megf8tm1Nju/Megf8+
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo |
Present |
Widespread |
1B
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Specimen
1C:
embryonic day 7.5; Megf8tm1Nju/Megf8 +
(more )
Specimen
1C:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Megf8tm1Nju/Megf8+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
Widespread |
1C
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Specimen
1D:
embryonic day 8.5; Megf8tm1Nju/Megf8 +
(more )
Specimen
1D:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Megf8tm1Nju/Megf8+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: embryo |
Weak |
Widespread |
1D
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Low level, broad expression is observed.
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Specimen
1E:
embryonic day 9.5; Megf8tm1Nju/Megf8 +
(more )
Specimen
1E:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Megf8tm1Nju/Megf8+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Weak |
Widespread |
1E
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Low level, broad expression is observed.
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Specimen
1F:
embryonic day 10.5; Megf8tm1Nju/Megf8 +
(more )
Specimen
1F:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Megf8tm1Nju/Megf8+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: embryo |
Weak |
Widespread |
1F
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Low level, broad expression is observed.
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Specimen
1H:
embryonic day 11.5; Megf8tm1Nju/Megf8 +
(more )
Specimen
1H:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Megf8tm1Nju/Megf8+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: heart |
Present |
Widespread |
1H
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Specimen
1I:
embryonic day 12.5; Megf8tm1Nju/Megf8 +
(more )
Specimen
1I:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Megf8tm1Nju/Megf8+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: heart |
Present |
Widespread |
1I
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Specimen
1J:
embryonic day 13.5; Megf8tm1Nju/Megf8 +
(more )
Specimen
1J:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Megf8tm1Nju/Megf8+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: heart |
Present |
Widespread |
1J
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Specimen
1K:
embryonic day 15.5; Megf8tm1Nju/Megf8 +
(more )
Specimen
1K:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Megf8tm1Nju/Megf8+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: heart |
Present |
Regionally restricted |
1K
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Specimen
1L:
embryonic day 8.5; Megf8tm1Nju/Megf8 +
(more )
Specimen
1L:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Megf8tm1Nju/Megf8+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: heart |
Present |
Single cells |
1L
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Specimen
1M:
embryonic day 10.5; Megf8tm1Nju/Megf8 +
(more )
Specimen
1M:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Megf8tm1Nju/Megf8+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: heart |
Present |
Single cells |
1M
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Specimen
1N:
embryonic day 12.5; Megf8tm1Nju/Megf8 +
(more )
Specimen
1N:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Megf8tm1Nju/Megf8+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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Specimen
1O:
embryonic day 12.5; Megf8tm1Nju/Megf8 +
(more )
Specimen
1O:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Megf8tm1Nju/Megf8+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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Specimen
1P:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Megf8tm1Nju/Megf8tm1Nju
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: embryo |
Present |
Widespread |
1P
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Specimen
1Q:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Megf8tm1Nju/Megf8tm1Nju
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: heart |
Present |
Widespread |
1Q
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Specimen
1R:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Megf8tm1Nju/Megf8tm1Nju
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: heart |
Present |
Widespread |
1R
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Analysis Tools