Meg3 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:82706 Croteau S, et al., Alternative splicing and imprinting control of the Meg3/Gtl2-Dlk1 locus in mouse embryos. Mamm Genome. 2003 Apr;14(4):231-41
Assay type:	RT-PCR
MGI Accession ID:	MGI:6450866
Gene symbol:	Meg3
Gene name:	maternally expressed 3

Probe:	Meg3 4F, Meg3 10R
Probe preparation:	labelled with P32
Visualized with:	Phosphorimaging
Assay notes:	These data are reported in Table 3. The primer set used in this assay amplifies exons 4-10; it generates products from nine splice variants. RFLP analysis and sequencing was used to determine which allele was responsible for observed expression of each isoform. Not all isoforms were analyzed in all tissues.

Results

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (c)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
MB 12 - fetus	E11.5	TS19: embryo	Present (b)	Present (d)	Not Specified	involves: C57BL/6 * MOLF/Ei		Not Specified	These embryos were the result of crossing (C57BL/6 x MOLF/Ei)F1 females to C57BL/6 males.
MB 12 - placenta	E11.5	TS19: extraembryonic component	Present (e)	Present (f)	Not Specified	involves: C57BL/6 * MOLF/Ei		Not Specified	These embryos were the result of crossing (C57BL/6 x MOLF/Ei)F1 females to C57BL/6 males.
MB 12 - adult muscle	P adult	TS28: skeletal muscle tissue	Present (g)	Absent (h)	Not Specified	involves: C57BL/6 * MOLF/Ei		Not Specified	This animal was the result of crossing (C57BL/6 x MOLF/Ei)F1 females to C57BL/6 males.
BM 12 - fetus	E11.5	TS19: embryo	Present (i)	Absent (j)	Not Specified	involves: C57BL/6 * MOLF/Ei		Not Specified	These embryos were the result of crossing C57BL/6 females to (C57BL/6 x MOLF/Ei)F1 males.
BM 12 - placenta	E11.5	TS19: extraembryonic component	Present (k)	Absent (l)	Not Specified	involves: C57BL/6 * MOLF/Ei		Not Specified	These embryos were the result of crossing C57BL/6 females to (C57BL/6 x MOLF/Ei)F1 males.

Notes:
(a) This band represents expression from the maternal allele.
(b) Expression was observed from the maternal (Molossinus) allele. Observed isoforms were: e (exons 4-5-6-7-8-9-10); f (exons 4-5-7-8-9-10); and h (exons 4-6-7-8-9-10). The j isoform (exons 4-5-7-8-10) was exclusively expressed from the paternal (C57BL/6) allele, i.e. it is not expressed from the maternal allele.
(c) This band represents expression from the paternal allele.
(d) The j isoform (exons 4-5-7-8-10) was exclusively expressed from the paternal (C57BL/6) allele. The other analyzed isoforms were not expressed paternally: e (exons 4-5-6-7-8-9-10); f (exons 4-5-7-8-9-10); and h (exons 4-6-7-8-9-10).
(e) Expression was observed from the maternal (Molossinus) allele. Isoforms e (exons 4-5-6-7-8-9-10) and f (exons 4-5-7-8-9-10) were exclusively maternally expressed. Isoforms h (exons 4-6-7-8-9-10) and i (exons 4-7-8-9-10) were biallelically expressed. Four other isoforms were exclusively paternally expressed: k (exons 4-5-6-7-8-10); j (exons 4-5-7-8-10); l (exons 4-5-7-8-10); and m (exons 4-7-8-10).
(f) Expression was observed from the paternal (C57BL/6) allele. Four isoforms were exclusively paternally expressed: k (exons 4-5-6-7-8-10); j (exons 4-5-7-8-10); l (exons 4-5-7-8-10); and m (exons 4-7-8-10). Isoforms h (exons 4-6-7-8-9-10) and i (exons 4-7-8-9-10) were biallelically expressed. Two other isoforms were exclusively maternally expressed: e (exons 4-5-6-7-8-9-10) and f (exons 4-5-7-8-9-10).
(g) Expression was observed from the maternal (Molossinus) allele. Observed isoforms were e (exons 4-5-6-7-8-9-10); f (exons 4-5-7-8-9-10); and g (exons 4-5-7-8-9-9a-10).
(h) No expression was observed from the paternal (C57BL/6) allele. Analyzed isoforms were e (exons 4-5-6-7-8-9-10); f (exons 4-5-7-8-9-10); and g (exons 4-5-7-8-9-9a-10).
(i) Expression was observed from the maternal (C57BL/6) allele. Observed isoforms were e (exons 4-5-6-7-8-9-10); f (exons 4-5-7-8-9-10); and h (exons 4-6-7-8-9-10).
(j) No expression was observed from the paternal (Molossinus) allele. Analyzed isoforms were: e (exons 4-5-6-7-8-9-10); f (exons 4-5-7-8-9-10); and h (exons 4-6-7-8-9-10).
(k) Expression was observed from the maternal (C57BL/6) allele. Observed isoforms were e (exons 4-5-6-7-8-9-10); f (exons 4-5-7-8-9-10); h (exons 4-6-7-8-9-10); i (exons 4-7-8-9-10); and j (exons 4-5-7-8-10).
(l) No expression was observed from the paternal (Molossinus) allele. Analyzed isoforms were: e (exons 4-5-6-7-8-9-10); f (exons 4-5-7-8-9-10); h (exons 4-6-7-8-9-10); i (exons 4-7-8-9-10); and j (exons 4-5-7-8-10).

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