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Gene Expression Data
Assay Details
Assay
Reference: J:82706 Croteau S, et al., Alternative splicing and imprinting control of the Meg3/Gtl2-Dlk1 locus in mouse embryos. Mamm Genome. 2003 Apr;14(4):231-41
Assay type: RT-PCR
MGI Accession ID: MGI:6450863
Gene symbol: Meg3
Gene name: maternally expressed 3
Probe: Meg3 1F, Meg3 4R
Probe preparation: labelled with P32
Visualized with: Phosphorimaging
Assay notes: These data are reported in Table 3. The primer set used in this assay amplifies exons 1-4; it generates products from two splice variants. RFLP analysis and sequencing was used to determine which allele was responsible for observed expression of each isoform.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (c) Size Not Specified (d) Size Not Specified (e) Amount Genetic BackgroundMutant Allele(s)Sex Note
MB 12 - fetus E11.5 TS19: embryo Absent (b) Absent (b) Present Present Not Specified involves: C57BL/6 * MOLF/Ei Not Specified These embryos were the result of crossing (C57BL/6 x MOLF/Ei)F1 females to C57BL/6 males.
MB 12 - placenta E11.5 TS19: extraembryonic component Absent (b) Absent (b) Present Present Not Specified involves: C57BL/6 * MOLF/Ei Not Specified These embryos were the result of crossing (C57BL/6 x MOLF/Ei)F1 females to C57BL/6 males.
BM 12 - fetus E11.5 TS19: embryo Present Not Applicable (f) Absent (b) Not Applicable (f) Not Specified involves: C57BL/6 * MOLF/Ei Not Specified These embryos were the result of crossing C57BL/6 females to (C57BL/6 x MOLF/Ei)F1 males.
BM 12 - placenta E11.5 TS19: extraembryonic component Present Not Applicable (f) Absent (b) Not Applicable (f) Not Specified involves: C57BL/6 * MOLF/Ei Not Specified These embryos were the result of crossing C57BL/6 females to (C57BL/6 x MOLF/Ei)F1 males.
Notes:
(a) This band represents expression of isoform b (exons 1-2-3-3b-4) from the C57BL/6 allele.
(b) Expression of this isoform from the paternally-derived allele is not seen.
(c) This band represents expression of isoform a (exons 1-2-2a-3-3b-4) from the C57BL/6 allele.
(d) This band represents expression of isoform b (exons 1-2-3-3b-4) from the Molossinus allele.
(e) This band represents expression of isoform a (exons 1-2-2a-3-3b-4) from the Molossinus allele.
(f) This isoform was not tested for this cross.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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