Dlk1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:82706 Croteau S, et al., Alternative splicing and imprinting control of the Meg3/Gtl2-Dlk1 locus in mouse embryos. Mamm Genome. 2003 Apr;14(4):231-41
Assay type:	RT-PCR
MGI Accession ID:	MGI:6450854
Gene symbol:	Dlk1
Gene name:	delta like non-canonical Notch ligand 1

Probe:	Dlk 1F, Dlk 5R
Probe preparation:	labelled with P32
Visualized with:	Phosphorimaging
Assay notes:	These data are reported in Table 2. The primer set used in this assay amplifies exons 1-5. There are two splice forms; the large form refers to the isoform with the unspliced exon 5, the small form refers to the isoform in which a 222 bp sequence from exon 5 is spliced out. RFLP analysis was used to determine which allele was responsible for observed expression.

Results

	Sample Information		Bands				Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (b)	Size Not Specified (c)	Size Not Specified (e)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
MB 12	E11.5	TS19: embryo	Present	Present	Absent (d)	Present (f)	Not Specified	involves: C57BL/6 * MOLF/Ei		Not Specified	These embryos were the result of crossing (C57BL/6 x MOLF/Ei)F1 females to C57BL/6 males.
BM 12	E11.5	TS19: embryo	Absent (d)	Absent (d)	Present	Present	Not Specified	involves: C57BL/6 * MOLF/Ei		Not Specified	These embryos were the result of crossing C57BL/6 females to (C57BL/6 x MOLF/Ei)F1 males.

Notes:
(a) This band represents expression of the small splice variant from the C57BL/6 allele.
(b) This band represents expression of the large splice variant from the C57BL/6 allele.
(c) This band represents expression of the small splice variant from the Molossinus allele.
(d) Expression of this isoform from the maternally-derived allele is not seen.
(e) This band represents expression of the large splice variant from the Molossinus allele.
(f) Expression of this isoform from the maternally-derived allele was seen, demonstrating a relaxation of imprinting in embryos derived from this cross in isoforms that contain the unspliced exon 5.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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