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Gene Expression Data
Assay Details
Assay
Reference: J:82706 Croteau S, et al., Alternative splicing and imprinting control of the Meg3/Gtl2-Dlk1 locus in mouse embryos. Mamm Genome. 2003 Apr;14(4):231-41
Assay type: RT-PCR
MGI Accession ID: MGI:6450852
Gene symbol: Dlk1
Gene name: delta like non-canonical Notch ligand 1
Probe: Dlk 4F, Dlk 5R
Probe preparation: labelled with P32
Visualized with: Phosphorimaging
Assay notes: The primer set used in this assay amplifies exons 4-5. RFLP analysis was used to determine which allele was responsible for observed expression.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (c) Amount Genetic BackgroundMutant Allele(s)Sex Note
BM 12 - not shown E11.5 TS19: embryo Absent (b) Present Not Specified involves: C57BL/6 * MOLF/Ei Not Specified These embryos were the result of crossing C57BL/6 females to (C57BL/6 x MOLF/Ei)F1 males.
BM 12 - not shown E11.5 TS19: extraembryonic component Absent (b) Present Not Specified involves: C57BL/6 * MOLF/Ei Not Specified These embryos were the result of crossing C57BL/6 females to (C57BL/6 x MOLF/Ei)F1 males.
MB 12 - not shown E11.5 TS19: embryo Present Present (d) Not Specified involves: C57BL/6 * MOLF/Ei Not Specified These embryos were the result of crossing (C57BL/6 x MOLF/Ei)F1 females to C57BL/6 males.
MB 12 - not shown E11.5 TS19: extraembryonic component Present Present (d) Not Specified involves: C57BL/6 * MOLF/Ei Not Specified These embryos were the result of crossing (C57BL/6 x MOLF/Ei)F1 females to C57BL/6 males.
Notes:
(a) This band represents expression of the C57BL/6 allele.
(b) Expression of this isoform from the maternally-derived allele resulting from this cross is not seen; 0% of the total RNA is transcribed it.
(c) This band represents expression of the Molossinus allele.
(d) There is a partial relaxation of imprinting in embryos derived from this cross; 12-50% of the total RNA for this isoform is transcribed from the maternally-derived allele.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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