Dlk1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:82706 Croteau S, et al., Alternative splicing and imprinting control of the Meg3/Gtl2-Dlk1 locus in mouse embryos. Mamm Genome. 2003 Apr;14(4):231-41
Assay type:	RT-PCR
MGI Accession ID:	MGI:6450751
Gene symbol:	Dlk1
Gene name:	delta like non-canonical Notch ligand 1

Probe:	Dlk 1F, Dlk 4R
Probe preparation:	labelled with P32
Visualized with:	Phosphorimaging
Assay notes:	The primer set used in this assay amplifies exons 1-4. RFLP analysis was used to determine which allele was responsible for observed expression.

Results

Image: 3B lower

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (b)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
BM 12 - U	Control
BM 12 - D	E11.5	TS19: embryo	Present	Absent (c)	Not Specified	involves: C57BL/6 * MOLF/Ei		Not Specified	These embryos were the result of crossing C57BL/6 females to (C57BL/6 x MOLF/Ei)F1 males.
MB 12 - U	Control
MB 12 - D	E11.5	TS19: embryo	Present (d)	Present	Not Specified	involves: C57BL/6 * MOLF/Ei		Not Specified	These embryos were the result of crossing (C57BL/6 x MOLF/Ei)F1 females to C57BL/6 males.
C57BL/6 - U	Control
C57BL/6 - D	E11.5	TS19: embryo	Not Applicable	Present	Not Specified	C57BL/6		Not Specified

Notes:
(a) This band represents expression of the Molossinus allele.
(b) This band represents expression of the C57BL/6 allele.
(c) Expression of this isoform from the maternally-derived allele resulting from this cross is not seen; 0% of the total RNA is transcribed it.
(d) There is a partial relaxation of imprinting in embryos derived from this cross; 12-50% of the total RNA for this isoform is transcribed from the maternally-derived allele.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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