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Gene Expression Data
Assay Details
Assay
Reference: J:223046 Rieusset A, et al., Stochastic loss of silencing of the imprinted Ndn/NDN allele, in a mouse model and humans with prader-willi syndrome, has functional consequences. PLoS Genet. 2013;9(9):e1003752
Assay type: RNA in situ
MGI Accession ID: MGI:6450512
Gene symbol: Ndn
Gene name: necdin, MAGE family member
Probe: Ndn probe5
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Horseradish peroxidase
Assay notes: A peroxidase conjugated anti-digoxigenin antibody was used to detect the Ndn hybridized riboprobe, visualized using tyramide signal amplification.
Results
Specimen 2A WT: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: preoptic area Present 2A WT ISH
Specimen 2A Ndn+m/-p: embryonic day 12.5; Ndntm1.1Mus/Ndn+ (more )
Note: Maternally derived allele.
Structure Level Pattern Image Note
TS20: preoptic area Weak Regionally restricted 2A Ndn ISH
Specimen 2B WT: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hypothalamus Present Regionally restricted 2B WT ISH Expression is in the supraoptic area.
Specimen 2B Ndn+m/-p: embryonic day 12.5; Ndntm1.1Mus/Ndn+ (more )
Note: Maternally derived allele.
Structure Level Pattern Image Note
TS20: hypothalamus Weak Regionally restricted 2B Ndn ISH Expression is in the supraoptic area.
Specimen 2C WT: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: thalamus Present 2C WT ISH
Specimen 2C Ndn+m/-p: embryonic day 12.5; Ndntm1.1Mus/Ndn+ (more )
Note: Maternally derived allele.
Structure Level Pattern Image Note
TS20: thalamus Weak Regionally restricted 2C Ndn ISH
Specimen 2D WT: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: pons Present 2D WT ISH
Specimen 2D Ndn+m/-p: embryonic day 12.5; Ndntm1.1Mus/Ndn+ (more )
Note: Maternally derived allele.
Structure Level Pattern Image Note
TS20: pons Weak Regionally restricted 2D Ndn ISH
Specimen 2E WT: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: dorsal root ganglion Present 2E WT ISH
Specimen 2E Ndn+m/-p: embryonic day 12.5; Ndntm1.1Mus/Ndn+ (more )
Note: Maternally derived allele.
Structure Level Pattern Image Note
TS20: dorsal root ganglion Weak Regionally restricted 2E Ndn ISH
Specimen not shown E12.5: embryonic day 12.5; Ndntm1.1Mus/Ndn+ (more )
Note: Maternally derived allele.
Structure Level Pattern Image Note
TS20: spinal cord Present
TS20: midbrain Present Regionally restricted Expression is in the tegmentum.
TS20: prethalamus Present Regionally restricted Expression is in the subthalamus.
TS20: cerebral cortex Absent
TS20: myotome Absent
TS20: tongue Absent
Specimen S2 A WT: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: preoptic area Present S2A WT ISH
Specimen S2 A Ndn-/-: embryonic day 12.5; Ndntm1.1Mus/Ndntm1.1Mus (more )
Structure Level Pattern Image Note
TS20: preoptic area Absent S2A Ndn-/- ISH
Specimen S2 B WT: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hypothalamus Present Regionally restricted S2B WT ISH Expression is in the supraoptic area.
Specimen S2 B Ndn-/-: embryonic day 12.5; Ndntm1.1Mus/Ndntm1.1Mus (more )
Structure Level Pattern Image Note
TS20: hypothalamus Absent S2B Ndn-/- ISH
Specimen S2 C WT: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: thalamus Present S2C WT ISH
Specimen S2 C Ndn-/-: embryonic day 12.5; Ndntm1.1Mus/Ndntm1.1Mus (more )
Structure Level Pattern Image Note
TS20: thalamus Absent S2C Ndn-/- ISH
Specimen S2 D WT: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: pons Present S2D WT ISH
Specimen S2 D Ndn-/-: embryonic day 12.5; Ndntm1.1Mus/Ndntm1.1Mus (more )
Structure Level Pattern Image Note
TS20: pons Absent S2D Ndn-/- ISH
Specimen S2 E WT: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: dorsal root ganglion Present S2E WT ISH
Specimen S2 E Ndn-/-: embryonic day 12.5; Ndntm1.1Mus/Ndntm1.1Mus (more )
Structure Level Pattern Image Note
TS20: dorsal root ganglion Absent S2E Ndn-/- ISH
Specimen S3 A WT: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: diencephalon Present S3A WT ISH Expression is in the preoptic area.
Specimen S3 A Ndn+m/-p: embryonic day 10.5; Ndntm1.1Mus/Ndn+ (more )
Note: Maternally derived allele.
Structure Level Pattern Image Note
TS17: diencephalon Absent S3A Ndn+m/-p ISH
Specimen S3 B WT: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted S3B WT ISH Expression is in the supraoptic area.
Specimen S3 B Ndn+m/-p: embryonic day 10.5; Ndntm1.1Mus/Ndn+ (more )
Note: Maternally derived allele.
Structure Level Pattern Image Note
TS17: diencephalon Absent S3B Ndn+m/-p ISH
Specimen S3 C WT: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted S3C WT ISH Expression is in the thalamus.
Specimen S3 C Ndn+m/-p: embryonic day 10.5; Ndntm1.1Mus/Ndn+ (more )
Note: Maternally derived allele.
Structure Level Pattern Image Note
TS17: diencephalon Absent S3C Ndn+m/-p ISH
Specimen S3 D WT: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted S3D WT ISH Expression is in the pons.
Specimen S3 D Ndn+m/-p: embryonic day 10.5; Ndntm1.1Mus/Ndn+ (more )
Note: Maternally derived allele.
Structure Level Pattern Image Note
TS17: hindbrain Absent S3D Ndn+m/-p ISH
Specimen S3 E WT: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: dorsal root ganglion Present S3E WT ISH
Specimen S3 E Ndn+m/-p: embryonic day 10.5; Ndntm1.1Mus/Ndn+ (more )
Note: Maternally derived allele.
Structure Level Pattern Image Note
TS17: dorsal root ganglion Absent S3E Ndn+m/-p ISH
Specimen S6 A WT: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: telencephalon Present Regionally restricted S6A WT ISH Expression is in the septum.
Specimen S6 A Ndn+m/-p: embryonic day 12.5; Ndntm2Stw/Ndn+ (more )
Note: Maternally derived allele.
Structure Level Pattern Image Note
TS20: telencephalon Absent S6A Ndn+m/-p ISH
Specimen S6 B WT: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: preoptic area Present S6B WT ISH
Specimen S6 B Ndn+m/-p: embryonic day 12.5; Ndntm2Stw/Ndn+ (more )
Note: Maternally derived allele.
Structure Level Pattern Image Note
TS20: preoptic area Absent S6B Ndn+m/-p ISH
Specimen S6 C WT: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: thalamus Present S6C WT ISH
Specimen S6 C Ndn+m/-p: embryonic day 12.5; Ndntm2Stw/Ndn+ (more )
Note: Maternally derived allele.
Structure Level Pattern Image Note
TS20: thalamus Absent S6C Ndn+m/-p ISH
Specimen S6 D WT: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: pons Present S6D WT ISH
Specimen S6 D Ndn+m/-p: embryonic day 12.5; Ndntm2Stw/Ndn+ (more )
Note: Maternally derived allele.
Structure Level Pattern Image Note
TS20: pons Absent S6D Ndn+m/-p ISH
Specimen S6 E WT: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: dorsal root ganglion Present S6E WT ISH
Specimen S6 E Ndn+m/-p: embryonic day 12.5; Ndntm2Stw/Ndn+ (more )
Note: Maternally derived allele.
Structure Level Pattern Image Note
TS20: dorsal root ganglion Absent S6E Ndn+m/-p ISH
Specimen not shown E14.5: embryonic day 14.5; Ndntm2Stw/Ndn+ (more )
Note: Maternally derived allele.
Structure Level Pattern Image Note
TS22: dorsal root ganglion Absent
TS22: pons Absent
TS22: preoptic area Absent
TS22: telencephalon septum Absent
TS22: thalamus Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory